331569 | Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem

331569
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      69011-20-7

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      1.02243.0001
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          Plastic ampoule
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          331569-1EA
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              Plastic ampoule 1 ea
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              Description
              OverviewDeveloped specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
              Catalogue Number331569
              Brand Family Calbiochem®
              Application Data
              In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
              References
              ReferencesMacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res. 48, 103.
              Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
              Product Information
              CAS number69011-20-7
              ATP CompetitiveN
              FormGray-brown suspension
              ReversibleN
              Applications
              Biological Information
              Primary TargetRemoval of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
              Physicochemical Information
              Cell permeableN
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              R PhraseR: 36

              Irritating to eyes.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Irritant
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsWhen the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              331569

              References

              Reference overview
              MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res. 48, 103.
              Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.

              Brochure

              Title
              Dyes and Stains Technical Bulletin
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision16-December-2010 RFH
              Application Data
              In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
              DescriptionDeveloped specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
              FormGray-brown suspension
              Recommended reaction conditions

              Ethidium bromide (EtBr) is the fluorescence dye most frequently used to stain nucleic acids in agarose and polyacrylamide gels. It intercalates primarily with double-stranded DNA to form an orange-red fluorescent complex under the influence of UV light. This interaction with nucleic acids makes ethidium bromide potentially mutagenic and is classified as toxic.

              Capacity

              The column capacity is at least 300 mg EtBr, corresponding to 30 L gel staining solution. The binding capacity of the cartridge is tested with a 10 mg/L EtBr solution of 1X TAE buffer; >99% of the dye was adsorbed up to the 300 mg limit as measured by fluorescence spectrometry.

              Flow Rate

              The flow rate of the adsorber column depends on the volume and height of the reservoir used for the staining solution and is a minimum of 200 ml/hr.

              Connecting the Adsorber Column

              The cartridge, as supplied, is sealed at both ends with small plastic caps (see figure 1). These caps should be removed prior to use and the cartridge connected to a reservoir containing the ethidium bromide solution to be adsorbed. A glass or plastic reservoir fitted with a stopcock or tubing clamp should be used to that the column can be switched on and off as required. To avoid any blocking of the frits with gel particles in the staining solutions, it is advisable to put some glass wool, filter paper, etc. in the connecting tube. If the flow rate of the adsorber column is distinctly lower than 1-2 drops per second, residual air bubbles must be removed by repeatedly short-squeezing the tubing above the cartridge. To guarantee a good flow rate throughout the lifespan of the cartridge, the formation of air bubbles in the system should be avoided, as these tend to hinder flow. The residual capacity of the column is easy to assess visually as the ethidium bromide moves through the column in the form of an increasingly dark red to black band. As long as this band has not reached the bottom of the column, the concentration of dye is still below the specified detection limit.

              Figure 1: Ethidium Bromide Adsorber

              In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.



              Disposal

              Once the capacity has been exhausted, the adsorber column should be disposed of according to local regulations.
              CAS number69011-20-7
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsWhen the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
              Toxicity Irritant
              ReferencesMacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res. 48, 103.
              Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.