374096 Eosinophil Peroxidase, Human

374096
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      374096-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewNative, human eosinophil peroxidase. Eosinophil peroxidase is a secreted hemoprotein that catalyzes oxidations of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase (Cat. No. 475911), EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of ROS (reactive oxygen species) and RNS (reactive nitrogen species).
          Catalogue Number374096
          Brand Family Calbiochem®
          SynonymsEPO
          References
          ReferencesAndreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
          Borelli, V., et al. 2003. Infect. Immun. 71, 605.
          Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
          McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
          Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
          Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
          Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
          Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.
          Product Information
          CAS number9003-99-0
          Unit of DefinitionOne unit is defined as the amount of enzyme that will cause an increase in absorbance of 1 at 470 nm per min at pH 7.0 and 25°C, calculated from the initial linear rate of reaction using guaiacol as substrate.
          EC number1.11.1.7
          FormLyophilized
          FormulationLyophilized from 5 mM phosphate buffer, pH 7.0.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV and HIV.
          Specific Activity≥ 270 units/mg protein (Guaiacol Assay Method)
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C). If solutions must be frozen the protein concentration should be >1 mg/ml and the activity determined following each thaw.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Eosinophil Peroxidase, Human SDS

          Title

          Safety Data Sheet (SDS) 

          Eosinophil Peroxidase, Human Certificates of Analysis

          TitleLot Number
          374096

          References

          Reference overview
          Andreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
          Borelli, V., et al. 2003. Infect. Immun. 71, 605.
          Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
          McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
          Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
          Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
          Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
          Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-July-2008 RFH
          SynonymsEPO
          DescriptionNative, human eosinophil peroxidase. Eosinophil peroxidase is a secreted hemoprotein that catalyzes the oxidation of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase (Cat. No. 475911), EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS).
          FormLyophilized
          FormulationLyophilized from 5 mM phosphate buffer, pH 7.0.
          SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV and HIV.
          CAS number9003-99-0
          EC number1.11.1.7
          Purity≥90% by SDS-PAGE
          Specific activity≥ 270 units/mg protein (Guaiacol Assay Method)
          Unit definitionOne unit is defined as the amount of enzyme that will cause an increase in absorbance of 1 at 470 nm per min at pH 7.0 and 25°C, calculated from the initial linear rate of reaction using guaiacol as substrate.
          SolubilityReconstitute in 400 µl sterile, distilled H₂O to yield a final concentration of ~1.7 µM.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C). If solutions must be frozen the protein concentration should be >1 mg/ml and the activity determined following each thaw.
          Toxicity Standard Handling
          ReferencesAndreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
          Borelli, V., et al. 2003. Infect. Immun. 71, 605.
          Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
          McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
          Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
          Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
          Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
          Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.