324715 | Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

324715
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324715-5UG
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          Plastic ampoule 5 μg
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          Description
          OverviewNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
          Catalogue Number324715
          Brand Family Calbiochem®
          References
          ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
          Product Information
          CAS number72561-05-8
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
          EC number3.4.21.50
          FormLyophilized
          FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
          Hygroscopic Hygroscopic
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Specific Activity≥200 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 20-36/37/38-42/43

          Harmful by inhalation.
          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation and skin contact.
          S PhraseS: 22-26-36

          Do not breathe dust.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324715

          References

          Reference overview
          Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

          Brochure

          Title
          Proteases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-February-2012 RFH
          DescriptionNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
          FormLyophilized
          FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
          Recommended reaction conditions
          Protocol 1. Dissolve the proteins to be sequenced in 25 mM Tris-HCl, pH 8.5, and 1 mM EDTA. For proteins that are difficult to solubilize, add urea, SDS, or guanidine-HCl to the digestion buffer prior to solubilization. If urea is used, add 20 mmol/l of methylamine. 2. The suggested working concentration is 1:20-1:100 (protease:protein by weight). 3. Incubate for 2-18 h at 37°C.
          CAS number72561-05-8
          EC number3.4.21.50
          Purity≥90% by SDS-PAGE
          Specific activity≥200 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
          SolubilityReconstitute in 50 µl of distilled H₂O.
          Storage +2°C to +8°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
          Toxicity Harmful
          ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

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          Categories

          Life Science Research > Proteins and Enzymes > Other Enzymes