324714 Endoproteinase Lys-C, Lysobacter enzymogenes

324714
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324714-3U
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          Glass bottle 3 u
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          Description
          OverviewNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide or ester bonds at the carboxylic side of lysine in peptides and proteins. Useful for sequence and structural analysis. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
          Catalogue Number324714
          Brand Family Calbiochem®
          References
          ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
          Product Information
          CAS number72561-05-8
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
          EC number3.4.21.50
          FormLyophilized
          Applications
          Biological Information
          Specific Activity≥150 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 20-36/37/38-42/43

          Harmful by inhalation.
          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation and skin contact.
          S PhraseS: 22-26-36

          Do not breathe dust.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324714

          References

          Reference overview
          Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

          Brochure

          Title
          Proteases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-June-2008 RFH
          DescriptionNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide or ester bonds at the carboxylic side of lysine in peptides and proteins. Useful for sequence and structural analysis. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Has an optimal pH of 8.5-8.8.
          FormLyophilized
          Recommended reaction conditions
          Protocol Method 1: 1. Add the endoproteinase to the substrate at a concentration of 1:100 (protease:protein by weight) in 100 mM ammonium bicarbonate, pH 9.0. 2. Incubate for 1-2 h at 37°C. 3. Add a second aliquot or protease equal to the initial amount added and continue to incubate at 37°C for 11 h. Method 2: 1. Alternatively, mix the endoproteinase with the substrate at a concentration of 1:100 by weight in 100 mM ammonium bicarbonate, pH 8.5, containing SDS (1 mg/ml). 2. Incubate for 2 h at 37°C. 3. Add a second aliquot of protease equal to the initial amount added and incubate at 37°C. 4. Remove aliquots at timed intervals and monitor the progress of the digestion by electrophoresis.
          CAS number72561-05-8
          EC number3.4.21.50
          Specific activity≥150 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
          SolubilityH₂O
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
          Toxicity Harmful
          ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.