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SCM018 | Embryoid Body (EB) Formation Medium

100 mL  
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      Key Specifications Table

      Key Applications
      Catalogue NumberSCM018
      DescriptionEmbryoid Body (EB) Formation Medium
      OverviewThe Embryoid Body (EB) Formation Medium has been optimized and qualified to support the formation of embryoid body. The medium can be used to form EB from hanging drops or suspension culture on non-adhesive Petri dishes. It has been validated for the differentiation of mouse ES cells into neural, adipogenic and cardiomyogenic cell lineages.
      Background InformationEmbryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Most protocols used to differentiate murine ES cells to a variety of cells involve an initial step in which the spontaneous differentiation of ES cells proceed through the formation of embryoid body (EB). EBs are formed by culturing ES cells in suspension culture in low attachment Petri dishes. The non-adhesive surfaces of Petri dishes promote the aggregation of ES cells into a tight ball of cells termed embryoid body. EBs are thought to help maintain an embryonic organization and closely mimic some of the earliest stages of embryonic development.
      Product Information
      ApplicationThe Embryoid Body Formation Medium (EB Media) has been optimized & qualified to support the formation of embryoid body.
      Key Applications
      • Cell Differentiation
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStored at -20°C until ready to use. Upon thawing, store at 2-8°C for up to one month.
      Packaging Information
      Material Size100 mL
      Transport Information
      Supplemental Information