MR-101-D | EmbryoMax® Advanced KSOM Embryo Medium

MR-101-D
50 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult, Embryo Culture
      Description
      Catalogue NumberMR-101-D
      Trade Name
      • Embryomax
      • Specialty Media
      DescriptionEmbryoMax® Advanced KSOM Embryo Medium
      Background InformationEmbryoMax Advanced KSOM Medium is a new, uniquely buffered modified version of KSOM that can be used as a single medium to harvest and culture preimplantation mouse embryos. The medium will maintain pH out on the bench top and also grow embryos in vitro, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture. The Advanced KSOM medium has been shown to produce a higher frequency of blastocyst formation in multiple mouse strains after 72 hours in culture when compared to standard KSOM medium.

      Traditionally, retrieving and then growing mouse embryos in vitro is usually a 2-step system. Embryos are commonly removed from the oviducts in a HEPES buffered medium (e.g. M2, FHM), which maintain their pH in air. The embryos are then transferred into a second medium which is bicarbonate buffered (e.g. M16, CZB, KSOM) and placed in a CO2 incubator in order to maintain the pH of the medium. Furthermore, the bicarbonate buffered medium needs to be pre-equilibrated in the incubator for several hours prior to receiving embryos to achieve optimal growth. The EmbryoMax Advanced KSOM Medium enables both embryo handling in atmospheric conditions and culturing in a CO2 incubator with a single medium. No medium pre-equilibration step is necessary for embryo harvest, but pre-equilibration is recommended for culture.
      References
      Product Information
      Applications
      ApplicationEmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
      Key Applications
      • Stem Cell Culture
      • Embryo Culture
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryos
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsCan be stored at -20°C up to the expiration date on the bottle.
      Once thawed it should be used within 2 weeks.

      Note: Our EmbryoMax® Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.
      Packaging Information
      Material Size50 mL
      Transport Information
      Supplemental Information
      Specifications