324681 | Elastase, Human Neutrophil

324681
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324681-100UG
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          Glass bottle 100 μg
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          324681-50UG
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              Plastic ampoule 50 μg
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              Description
              OverviewNative elastase from human neutrophil. A serine protease that degrades elastin, collagen, and proteoglycans. Its overexpression has been implicated in the development of pulmonary emphysema, rheumatoid arthritis, and cystic fibrosis.
              Catalogue Number324681
              Brand Family Calbiochem®
              SynonymsPMN Elastase
              References
              ReferencesSiedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
              Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
              Selak, M.A. 1992. Thromb. Haemost. 68, 570.
              Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.
              Product Information
              CAS number9004-06-2
              Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=454454">Cat. No. 454454</a>) per min at 25°C, pH 8.0.
              EC number3.4.21.37
              FormLyophilized solid
              FormulationSalt-free.
              Applications
              Biological Information
              PurityMajor band by SDS-PAGE
              SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
              Specific Activity≥20 units/mg protein.
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSJX7069000
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage -20°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              324681

              References

              Reference overview
              Siedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
              Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
              Selak, M.A. 1992. Thromb. Haemost. 68, 570.
              Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.

              Brochure

              Title
              Proteases Technical Bulletin

              Citations

              Title
            • Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660.
            • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
            • Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.
            • Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision02-May-2008 RFH
              SynonymsPMN Elastase
              DescriptionNative elastase from human neutrophil. Degrades collagen, elastin, and proteoglycans. Elastase has been implicated in the development of pulmonary emphysema and rheumatoid arthritis.
              FormLyophilized solid
              FormulationSalt-free.
              Recommended reaction conditions
              Human Neutrophil Elastase Protocol 1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl. 2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val-pNA, Cat. No. 324696) at 50 mM in DMSO. 3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well. 4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution. 5. Add enzyme to assay mixture, mix, and incubate at 25°C. 6. Monitor the change in absorbance at 410 nm. Use an ε of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.
              SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
              CAS number9004-06-2
              RTECSJX7069000
              EC number3.4.21.37
              PurityMajor band by SDS-PAGE
              Specific activity≥20 units/mg protein.
              Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.
              SolubilityReconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.
              Storage -20°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
              Toxicity Standard Handling
              Merck USA index14, 3535
              ReferencesSiedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
              Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
              Selak, M.A. 1992. Thromb. Haemost. 68, 570.
              Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.
              Citation
            • Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660.
            • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
            • Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.