L6143-BC Easycoll, Density 1.124 g/ml

L6143-BC
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      L6143
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          100 ml
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          Description
          OverviewThe basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Our ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (cat. no. L6143/5) can conveniently be prepared with PBS (cat. no. L1825).

          Separating solutions
          Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

          Easycoll
          The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Biochrom AG ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (cat. no. L6143/5) can conveniently be prepared with PBS (cat. no. L1825).

          Production of Easycoll with different densities
          The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value.
          But you can also adjust a required density yourself by diluting Easycoll correspondingly.
          The table below shows an example for different densities. All amounts are given for a temperature of +20 °C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions might influence agglomeration and hence separation of cell clusters.
          Tab. 37: Instructions on how to dilute the stock solution of Easycoll (density 1.124 g/ml; cat. no. L6143/L6145) with PBS (cat. no. L1825/0)
          See Table
          Preparation of solutions with other densities can be made by isoosmolar dilution according to the following formula:
          See figure
          For the dilution we recommend the use of PBS without Ca2+, Mg2+, since both ions can influence the agglomeration of cells and consequently cell separation.
          Specifications:

          Sterility: Pass
          pH: 7.02-7.62
          Osmolality: 0.281-0.310 Osm/kg H20
          Density: 1.123-1.125 g/ml
          Color: Light Yellow
          Appearance: Clear

          Catalogue NumberL6143-BC
          Brand Family Calbiochem®
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          Ship Code Blue Ice Only
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
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