17-701 EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

17-701
12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      RIP
      Description
      Catalogue Number17-701
      Brand Family Upstate
      Trade Name
      • EZ-Magna RIP
      • Upstate
      DescriptionEZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
      OverviewRNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

      Features & Benefits:
      -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
      -RNAse inhibitors and RNAse-free reagents
      -Positive and negative controls
      Alternate Names
      • Magnetic RIP kit
      Background InformationGene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
      products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
      Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
      References
      Product Information
      Components
      • Magnetic Beads Protein A/G
      • RIP Wash Buffer
      • RIP Lysis Buffer
      • 0.5 M EDTA
      • 10% SDS
      • Salt Solution I
      • Salt Solution II
      • Precipitate Enhancer
      • Normal Mouse IgG
      • Rabbit IgG Purified
      • Protease Inhibitor Cocktail 200X
      • RNase Inhibitor
      • Proteinase K (10 mg/mL)
      • Nuclease free water
      • Positive Control Antibody (Anti-SNRNP70)
      • Control Primers
      PresentationThree boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
      Includes positive control antibody and control primers.
      Applications
      ApplicationRNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads. Control primers included.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
      Packaging Information
      Material Size12 assays
      Material PackageRIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewApplicationPub Med ID
      LncRNA TUG1 sponges miR-204-5p to promote osteoblast differentiation through upregulating Runx2 in aortic valve calcification.
      Yu, C; Li, L; Xie, F; Guo, S; Liu, F; Dong, N; Wang, Y
      Cardiovasc Res  114  168-179  2018

      Show Abstract
      29016735 29016735
      Down-regulation of LncRNA CCAT1 enhances radiosensitivity via regulating miR-148b in breast cancer.
      Lai, Y; Chen, Y; Lin, Y; Ye, L
      Cell Biol Int  42  227-236  2018

      Show Abstract
      29024383 29024383
      High-content hydrogen water-induced downregulation of miR-136 alleviates non-alcoholic fatty liver disease by regulating Nrf2 via targeting MEG3.
      Wang, X; Wang, J
      Biol Chem  399  397-406  2018

      Show Abstract
      29261513 29261513
      Dual regulation of HMGB1 by combined JNK1/2-ATF2 axis with miR-200 family in nonalcoholic steatohepatitis in mice.
      Chen, X; Ling, Y; Wei, Y; Tang, J; Ren, Y; Zhang, B; Jiang, F; Li, H; Wang, R; Wen, W; Lv, G; Wu, M; Chen, L; Li, L; Wang, H
      FASEB J  32  2722-2734  2018

      Show Abstract
      29298863 29298863
      LncRNA GAS5 regulates ischemic stroke as a competing endogenous RNA for miR-137 to regulate the Notch1 signaling pathway.
      Chen, F; Zhang, L; Wang, E; Zhang, C; Li, X
      Biochem Biophys Res Commun  496  184-190  2018

      Show Abstract
      29307821 29307821
      The long non-coding RNA ENST00000547547 reduces 5-fluorouracil resistance of colorectal cancer cells via competitive binding to microRNA-31.
      Li, J; Li, X; Cen, C; Ai, X; Lin, C; Hu, G
      Oncol Rep  39  217-226  2018

      Show Abstract
      29115526 29115526
      Long non-coding RNA TCONS_00041960 enhances osteogenesis and inhibits adipogenesis of rat bone marrow mesenchymal stem cell by targeting miR-204-5p and miR-125a-3p.
      Shang, G; Wang, Y; Xu, Y; Zhang, S; Sun, X; Guan, H; Zhao, X; Wang, Y; Li, Y; Zhao, G
      J Cell Physiol  233  6041-6051  2018

      Show Abstract
      29319166 29319166
      LncRNA myocardial infarction-associated transcript (MIAT) contributed to cardiac hypertrophy by regulating TLR4 via miR-93.
      Li, Y; Wang, J; Sun, L; Zhu, S
      Eur J Pharmacol  818  508-517  2018

      Show Abstract
      29157986 29157986
      Long non-coding RNA ZFAS1 sponges miR-484 to promote cell proliferation and invasion in colorectal cancer.
      Xie, S; Ge, Q; Wang, X; Sun, X; Kang, Y
      Cell Cycle  17  154-161  2018

      Show Abstract
      29179614 29179614
      Nkx2-2as Suppression Contributes to the Pathogenesis of Sonic Hedgehog Medulloblastoma.
      Zhang, Y; Wang, T; Wang, S; Xiong, Y; Zhang, R; Zhang, X; Zhao, J; Yang, AG; Wang, L; Jia, L
      Cancer Res  78  962-973  2018

      Show Abstract
      29229597 29229597

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

      User Guides

      Title
      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits