17-10086 | EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit

17-10086
22 assays  Kit capacity: 22 chromatin immunoprecipitation assays
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Special Offers

      For target-specific spike-in controls that make ChIP experiments more quantitative and accurate, Click on the Related Product & Applications tab above.
      Description
      Catalogue Number17-10086
      Trade Name
      • Magna ChIP
      DescriptionEZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit
      OverviewUnlike standard ChIP protocols that can be laborious and time consuming, the Magna ChIP kit protocol can reduce the amount of time required to perform a ChIP experiment from three days to one. Additionally, the smaller Magna ChIP reaction volume increases the relative concentration of the antibody enabling the ChIP reaction to be performed with reduced amounts of both antibody and sheared chromatin. Finally because this kit uses a blend of protein A and protein G beads, a wider range of antibody isotypes can be used than A or G alone. This allows a wider variety of antibodies to be used and avoids the need to purchase separate kits for protein A and protein G based immunoprecipitation. Because Magna ChIP kits use paramagnetic beads they are compatible with automated high throughput platforms, thus allowing a large number of ChIP reactions to be carried out simultaneously. Features & Benefits:
      • Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
      • Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
      • Negative and positive control antibodies and control primer set to simplify validation of experimental procedure
      • Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
      • Complete kit with all required reagents for reliable and reproducible results
      • Compatible with ChIPAb+ validated antibody and primer sets

      Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
      Alternate Names
      • Magnetic ChIP Kit
      Background InformationChromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
      Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
      References
      Product Information
      Components
      • Magnetic Protein A/G Beads
      • ChIP Dilution Buffer
      • Low Salt Wash Buffer
      • High Salt Wash Buffer
      • LiCl Wash Buffer
      • TE Buffer
      • Cell Lysis Buffer
      • Nuclear Lysis Buffer
      • ChIP Elution Buffer (w/o Proteinase K)
      • Proteinase K
      • RNase A
      • 10X Glycine
      • 10X PBS
      • Protease Inhibitor Cocktail II
      • Anti-RNA polymerase II, clone CTD4H8
      • Normal Mouse IgG
      • Control Primers
      • Spin Filters
      • Collection Tubes
      • Bind Reagent A
      • Wash Reagent B
      • Elution Reagent C
      PresentationTwo boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
      Applications
      ApplicationSingle day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A/G beads. Control primers included.
      Biological Information
      Analytes Available
      • Protein A+G
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
      Packaging Information
      Material Size22 assays
      Material PackageKit capacity: 22 chromatin immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      An Introduction to Antibodies and Their Applications
      New Products: Volume 3, 2012
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Technical Info

      Title
      Getting started with ChIP-seq: experimental design to data analysis
      Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

      User Guides

      Title
      EZ-Magna ChIP™ A/G

      Related Products & Applications

      Related Products

      Catalog Number Description  
      17-10501 AccuChIP™ Dimethyl-Histone H3 (Lys27) Chromatin Immunoprecipitation Internal Control Show Pricing & Availability
      17-10502 AccuChIP™ Trimethyl-Histone H3 Internal Control(Lys27) Chromatin Immunoprecipitation Show Pricing & Availability
      17-10503 AccuChIP™ Trimethyl-Histone H3 (Lys36) Chromatin Immunoprecipitation Internal Control Show Pricing & Availability
      17-10504 AccuChIP™ Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control Show Pricing & Availability
      17-10505 AccuChIP™ Trimethyl-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control Show Pricing & Availability
      17-10506 AccuChIP™ Acetyl-Histone H3 (Lys9) Chromatin Immunoprecipitation Internal Control Show Pricing & Availability

      Related Products By: Application Facete

      Related Products By: Brand Facete

      Categories

      Life Science Research > Kits & Assays > ChIP Kits > Kits and Assays
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Kits & Beads