SCM004 ENStem-A™ Neural Expansion Medium

SCM004
500 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCM004
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionENStem-A™ Neural Expansion Medium
      OverviewENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.
      Materials Required but Not Delivered• L-Glutamine (Cat. No. TMS-002-C)
      • ENStem-A™ Human Neural Progenitor Expansion Kit (Cat. No. SCR055)
      • ENStem-A™ Neural Freezing Medium (Cat. No. SCM011)
      • ENStem-A™ Neuronal Differentiation Medium (Cat. No. SCM017)
      • Poly-L-ornithine hydrobromide (Sigma Part No. P3655)
      • Mouse Laminin (Sigma Part No. L-2020)
      • Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C)
      • Accutase™ (Cat. No. SCR005)
      • Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B)
      • Tissue culture-ware
      • Hemacytometer
      • Microscope
      References
      Product Information
      Components
      • ENStem-A Neural Expansion Medium, 500 mL (Part No. SCM004a)
      • FGF-2, 50ug, lyophilized (Part No. GF003)
      Formulation
      Applications
      ApplicationThe ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture & expansion of ENStem-A Human Neural Progenitor Cells.
      Key Applications
      • Stem Cell Culture
      Application NotesCell culture protocols for use with ENStem-A™ Human Neural Progenitor Cells are available in the datasheet that accompanies the ENStem-A™ Human Neural Progenitor Expansion Kit (Cat. No. SCR055).
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of medium is tested for the optimal cell growth and proliferation of ENStem-ATM Human Neural Progenitor Cells.
      Sterility Testing: Negative
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsENStem-ATM Neural Expansion Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the expansion medium. Thawed should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.

      FGF-2 (10 μg, lyophilized) should be reconstituted with 100 μL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 μg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ENStem-A™ Neural Expansion Medium SDS

      Title

      Safety Data Sheet (SDS) 

      ENStem-A™ Neural Expansion Medium Certificates of Analysis

      TitleLot Number
      ENStem-A TM Neural Expansion Medium -27843012784301
      ENStem-ATM Neural Expansion Medium2443044
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 21366262136626
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 21421792142179
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 21488882148888
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 23635632363563
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 23821642382164
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 23910312391031
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 24392052439205
      ENStem-ATM Neural Expansion Medium For ENStem-ATM Human Neural Progenitors - 24425472442547

      References

      Reference overviewPub Med ID
      Contralaterally transplanted human embryonic stem cell-derived neural precursor cells (ENStem-A) migrate and improve brain functions in stroke-damaged rats.
      Chang, Da-Jeong, et al.
      Exp. Mol. Med., 45: e53 (2013)  2013

      Show Abstract
      24232252 24232252

      Technical Info

      Title
      ENStem-A Adherent Human Neural Progenitors: A New Source of Primary Human Neural Cells
      Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

      Data Sheet

      Title
      ENStem-A™ Neural Expansion Medium For ENStem-A™ Human Neural Progenitors - Data Sheet

      Related Products & Applications

      Accessories

      Catalog Number Description  
      SCR055 ENStem Human Neural Progenitor Expansion Kit Show Pricing & Availability

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Stem Cell Culture Media