324831 | EGF, Human, Recombinant, E. coli

324831
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324831-200UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 200 μg
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          Description
          OverviewRecombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis.
          Catalogue Number324831
          Brand Family Calbiochem®
          SynonymsEpidermal Growth Factor, Human, Recombinant, E. coli
          References
          ReferencesMerlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
          Yoshida, T., et al. 1993. Brain Res. Dev. 76, 147.
          Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 415.
          Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
          Downward, J., et al. 1984. Nature 311, 483.
          Gray, A., et al. 1983. Nature 303, 722.
          Scott, J., et al. 1983. Science 221, 236.
          Cohen, S. 1960. Proc. Natl. Acad. Sci. USA 46, 302.
          Product Information
          CAS number62229-50-9
          FormSolid
          FormulationLyophilized from sterile-filtered PBS.
          Applications
          Biological Information
          Biological activityED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: <1.0 EU/μg EGF
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSKB4518000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324831

          References

          Reference overview
          Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
          Yoshida, T., et al. 1993. Brain Res. Dev. 76, 147.
          Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 415.
          Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
          Downward, J., et al. 1984. Nature 311, 483.
          Gray, A., et al. 1983. Nature 303, 722.
          Scott, J., et al. 1983. Science 221, 236.
          Cohen, S. 1960. Proc. Natl. Acad. Sci. USA 46, 302.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-May-2008 RFH
          SynonymsEpidermal Growth Factor, Human, Recombinant, E. coli
          DescriptionRecombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to native human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Binding of EGF to its 170 kDa transmembrane receptor results in dimerization of the receptor, activation of the intrinsic receptor tyrosine kinase, and initiation of the complex signal transduction cascade that leads to cell proliferation and/or differentiation. Acts as a survival factor in preventing apoptosis.
          FormSolid
          FormulationLyophilized from sterile-filtered PBS.
          CAS number62229-50-9
          RTECSKB4518000
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: <1.0 EU/μg EGF
          Biological activityED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
          SolubilityReconstitute to a concentration of ≥10 µg/ml in 10 mM acetic acid containing ≥0.1% HSA or BSA.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
          Toxicity Standard Handling
          Merck USA index14, 3527
          ReferencesMerlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
          Yoshida, T., et al. 1993. Brain Res. Dev. 76, 147.
          Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 415.
          Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
          Downward, J., et al. 1984. Nature 311, 483.
          Gray, A., et al. 1983. Nature 303, 722.
          Scott, J., et al. 1983. Science 221, 236.
          Cohen, S. 1960. Proc. Natl. Acad. Sci. USA 46, 302.

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          Categories

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          Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors