AP182B Donkey Anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed

AP182B
500 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP182B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDonkey Anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      References
      Product Information
      FormatBiotin
      PresentationLyophilized. Buffer = 0.01 M Sodium Phosphate, 0.15 M NaCl, pH 7.1 with 15 mg/mL BSA, and 0.1% sodium azide.

      RECONSTITUTION:

      Reconstitute with 500 μL of sterile distilled water.
      Applications
      ApplicationDonkey anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed detects level of Donkey Rabbit IgG & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesEIA: 1:50,000-1:100,000 Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000. Immunohistochemistry: 1:500-1:5,000 Flow cytometry: 1:200-1:1,000 Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000 Optimal working dilutions must be determined by end user.
      Biological Information
      HostDonkey
      SpecificityRabbit IgG
      Species Reactivity
      • Rabbit
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED2483204
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED - 31874723187472
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING - 32059373205937
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY3137093
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY2939727
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY2960661
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY2886878
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY2896421
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - 23733022373302
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - 20350542035054

      References

      Reference overviewPub Med ID
      The excitatory synaptic transmission of the nucleus of solitary tract was potentiated by chronic myocardial infarction in rats.
      Li, J; Zhang, MM; Tu, K; Wang, J; Feng, B; Zhang, ZN; Lei, J; Li, YQ; Du, JQ; Chen, T
      PloS one  10  e0118827  2015

      Show Abstract
      25756354 25756354
      Effects of maternal exposure to ultrafine carbon black on brain perivascular macrophages and surrounding astrocytes in offspring mice.
      Onoda, A; Umezawa, M; Takeda, K; Ihara, T; Sugamata, M
      PloS one  9  e94336  2014

      Show Abstract
      24722459 24722459
      Long-term exercise is a potent trigger for ΔFosB induction in the hippocampus along the dorso-ventral axis.
      Nishijima, T; Kawakami, M; Kita, I
      PloS one  8  e81245  2013

      Show Abstract
      24282574 24282574
      Gene expression changes in the olfactory bulb of mice induced by exposure to diesel exhaust are dependent on animal rearing environment.
      Yokota, S; Hori, H; Umezawa, M; Kubota, N; Niki, R; Yanagita, S; Takeda, K
      PloS one  8  e70145  2013

      Show Abstract
      23940539 23940539
      Corticotropin-releasing factor antagonist reduces activation of noradrenalin and serotonin neurons in the locus coeruleus and dorsal raphe in the arousal response accompanied by yawning behavior in rats.
      Natsuko Kubota,Seiichiro Amemiya,Chiharu Motoki,Tomomi Otsuka,Takeshi Nishijima,Ichiro Kita
      Neuroscience research  72  2012

      Show Abstract
      22285921 22285921
      Central administration of aminoprocalcitonin inhibits food intake and stimulates the hypothalamic-pituitary-adrenal axis in rats via the corticotrophin-releasing factor system.
      E Tavares,R Maldonado,A Garcia-Martinez,F J Miñano
      Journal of neuroendocrinology  24  2012

      Show Abstract
      22372932 22372932
      Maternal exposure to carbon black nanoparticle increases collagen type VIII expression in the kidney of offspring.
      Umezawa M, Kudo S, Yanagita S, Shinkai Y, Niki R, Oyabu T, Takeda K, Ihara T, Sugamata M.
      The Journal of toxicological sciences  36  461-8  2011

      Show Abstract
      21804310 21804310
      Quantification of pluripotency transcription factor levels in embryonic stem cells by flow cytometry.
      Nicola Festuccia,Ian Chambers
      Current protocols in stem cell biology  Chapter 1  2011

      Show Abstract
      22135081 22135081
      Effects of negative air ions on activity of neural substrates involved in autonomic regulation in rats.
      Satoko Suzuki, Shinya Yanagita, Seiichiro Amemiya, Yumi Kato, Natsuko Kubota, Tomoo Ryushi, Ichiro Kita
      International journal of biometeorology  52  481-9  2008

      Show Abstract
      18188611 18188611
      Effects of spontaneous and forced running on activation of hypothalamic corticotropin-releasing hormone neurons in rats.
      Shinya Yanagita,Seiichiro Amemiya,Satoko Suzuki,Ichiro Kita
      Life sciences  80  2007

      Show Abstract
      17067638 17067638

      Data Sheet

      Title
      Donkey anti-Rabbit IgG, Biotin-SP-Conjugated, Species Adsorbed: Hu, Ms, Rt, Bov, Gt, Sh, Chk, GP, Eq - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies