AP182R Donkey Anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed

AP182R
500 µg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP182R
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDonkey Anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      References
      Product Information
      FormatRhodamine (TRITC)
      PresentationLyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

      RECONSTITUTION:
      Reconstitute with 500 μL of sterile distilled water.

      RHODAMINE/PROTEIN:
      Approximately 0.52 (A550/A280)
      Applications
      ApplicationDonkey anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in IF.
      Key Applications
      • Immunofluorescence
      Application NotesSuggested dilution for most applications: 1:50-1:200

      Optimal working dilutions must be determined by end user.
      Biological Information
      HostDonkey
      SpecificityRabbit IgG

      WAVELENGTH:
      Absorption peak = 550nm, Emission peak = 570 nm.
      Species Reactivity
      • Rabbit
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, RHODAMINE CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY -
      RHODAMINE CONJUGATED AFFINITY PURIFIED SECONDARY ANTIBODY - 21365762136576
      RHODAMINE CONJUGATED AFFINITY PURIFIED SECONDARY ANTIBODY2883549
      RHODAMINE CONJUGATED AFFINITY PURIFIED SECONDARY ANTIBODY3049530
      RHODAMINE CONJUGATED AFFINITY PURIFIED SECONDARY ANTIBODY2934595

      References

      Reference overviewPub Med ID
      The effects of NAD+ on apoptotic neuronal death and mitochondrial biogenesis and function after glutamate excitotoxicity.
      Wang, X; Li, H; Ding, S
      International journal of molecular sciences  15  20449-68  2014

      Show Abstract
      25387075 25387075
      A mutation in the nuclear pore complex gene Tmem48 causes gametogenesis defects in skeletal fusions with sterility (sks) mice.
      Akiyama, K; Noguchi, J; Hirose, M; Kajita, S; Katayama, K; Khalaj, M; Tsuji, T; Fairfield, H; Byers, C; Reinholdt, L; Ogura, A; Kunieda, T
      The Journal of biological chemistry  288  31830-41  2013

      Show Abstract
      24045954 24045954
      Prognostic value of LYVE-1-positive lymphatic vessel in tongue squamous cell carcinomas.
      Naoyuki Matsumoto,Shotaro Mukae,Hirotaka Tsuda,Akihito Sawada,Yasuhiro Okazaki,Koichi Nagai,Yuri Akishima-Fukasawa,Hidero Ohki,Yukio Ishikawa,Toshiharu Ishii,Kazuo Komiyama
      Anticancer research  30  2010

      Show Abstract
      20651332 20651332
      Integrins mediate adherence and migration of T lymphocytes on human peritoneal mesothelial cells.
      Wang, HH; Lee, TY; Lin, CY
      Kidney international  74  808-16  2008

      Show Abstract
      18614994 18614994
      Differential distribution of orexin-A-like and orexin receptor 1 (OX1R)-like immunoreactivities in the Xenopus pituitary.
      H Suzuki, Y Takemoto, T Yamamoto
      Tissue cell  39  423-30  2007

      Show Abstract
      17897692 17897692
      Activated epidermal growth factor receptor induces integrin alpha2 internalization via caveolae/raft-dependent endocytic pathway.
      Ning, Y; Buranda, T; Hudson, LG
      The Journal of biological chemistry  282  6380-7  2007

      Show Abstract
      17179151 17179151
      Increase of Cardiotrophin-1 immunoreactivity in regenerating and overloaded but not denervated muscles of rats.
      Junji Nishikawa, Kunihiro Sakuma, Yoshihide Sorimachi, Kanji Yoshimoto, Masahiro Yasuhara
      Neuropathology : official journal of the Japanese Society of Neuropathology  25  54-65  2005

      Show Abstract
      15822819 15822819
      Spontaneous regeneration of the corticospinal tract after transection in young rats: a key role of reactive astrocytes in making favorable and unfavorable conditions for regeneration.
      T Iseda, T Nishio, S Kawaguchi, M Yamanoto, T Kawasaki, S Wakisaka
      Neuroscience  126  365-74  2004

      Show Abstract
      15207354 15207354
      Excitotoxic degeneration of hypothalamic orexin neurons in slice culture.
      Hiroshi Katsuki, Akinori Akaike
      Neurobiology of disease  15  61-9  2004

      Show Abstract
      14751771 14751771
      Association of dystroglycan and laminin-2 coexpression with myelinogenesis in peripheral nerves.
      Toshihiro Masaki, Kiichiro Matsumura, Fumiaki Saito, Hiroki Yamada, Susumu Higuchi, Keiko Kamakura, Hiroshi Yorifuji, Teruo Shimizu
      Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan  36  221-39  2003

      Show Abstract
      16228655 16228655

      Data Sheet

      Title
      RHODAMINE CONJUGATED AFFINITY PURIFIED SECONDARY ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies