AP182P Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed

AP182P
500 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP182P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDonkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      References
      Product Information
      FormatHRP
      PresentationLyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

      RECONSTITUTION:

      Reconstitute with 500 μL of sterile distilled water.
      Applications
      ApplicationDonkey anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesEIA and Western blots: 1:5,000-1:100,000.

      Immunohistochemistry: 1:500-1:5,000.

      Western blotting with ECL substrates: 1:10,000-1:200,000.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostDonkey
      SpecificityRabbit IgG
      Species Reactivity
      • Rabbit
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

      WARNING:

      Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed SDS

      Title

      Safety Data Sheet (SDS) 

      Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed Certificates of Analysis

      TitleLot Number
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, PEROXIDASE CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - 24651382465138
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, PEROXIDASE CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - 21385472138547
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, PEROXIDASE CONJUGATED -28868792886879
      DONKEY ANTI-RABBIT IGG - 32164863216486
      DONKEY ANTI-RABBIT IGG - 32305203230520
      DONKEY ANTI-RABBIT IGG - 33137563313756
      DONKEY ANTI-RABBIT IGG -25490082549008
      DONKEY ANTI-RABBIT IGG -26048132604813
      DONKEY ANTI-RABBIT IGG -26650352665035
      DONKEY ANTI-RABBIT IGG -26984242698424

      References

      Reference overviewApplicationPub Med ID
      Bile acids induce hepatic differentiation of mesenchymal stem cells.
      Sawitza, I; Kordes, C; Götze, S; Herebian, D; Häussinger, D
      Scientific reports  5  13320  2015

      Show Abstract
      26304833 26304833
      The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines.
      Kobayashi, K; Sakurai, K; Hiramatsu, H; Inada, K; Shiogama, K; Nakamura, S; Suemasa, F; Kobayashi, K; Imoto, S; Haraguchi, T; Ito, H; Ishizaka, A; Tsutsumi, Y; Iba, H
      Scientific reports  5  8428  2015

      Show Abstract
      Western Blotting25673149 25673149
      Possible role of ZPAC, zygote-specific proteasome assembly chaperone, during spermatogenesis in the mouse.
      Shimizu, N; Ueno, K; Kurita, E; Shin, SW; Nishihara, T; Amano, T; Anzai, M; Kishigami, S; Kato, H; Mitani, T; Hosoi, Y; Matsumoto, K
      The Journal of reproduction and development  60  179-86  2014

      Show Abstract
      24583807 24583807
      GSE is a maternal factor involved in active DNA demethylation in zygotes.
      Hatanaka, Y; Shimizu, N; Nishikawa, S; Tokoro, M; Shin, SW; Nishihara, T; Amano, T; Anzai, M; Kato, H; Mitani, T; Hosoi, Y; Kishigami, S; Matsumoto, K
      PloS one  8  e60205  2013

      Show Abstract
      Western Blotting23560077 23560077
      Placental melatonin production and melatonin receptor expression are altered in preeclampsia: new insights into the role of this hormone in pregnancy.
      Dave Lanoix,Pascale Guérin,Cathy Vaillancourt
      Journal of pineal research  53  2012

      Show Abstract
      22686298 22686298
      Stability of reference proteins in human placenta: general protein stains are the benchmark.
      D Lanoix,J St-Pierre,A-A Lacasse,M Viau,J Lafond,C Vaillancourt,A A Lacasse
      Placenta  33  2012

      Show Abstract
      22244735 22244735
      Fate mapping of the mouse prosencephalic neural plate.
      T Inoue, S Nakamura, N Osumi
      Developmental biology  219  373-83  2000

      Show Abstract
      10694429 10694429

      Data Sheet

      Title
      DONKEY ANTI-RABBIT IGG AFFINITY PURIFIED, PEROXIDASE CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies