A part of MilliporeSigma

AP192B | Donkey Anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed

500 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information
      Catalogue NumberAP192B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDonkey Anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      Product Information
      PresentationLyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA and 0.01% thimerosal.


      Reconstitute with 500 μL of sterile distilled water.
      ApplicationDonkey anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed detects level of Donkey Mouse IgG & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesEIA and Western blots: 1:5,000-1:100,000.

      Immunohistochemistry: 1:500-1:5,000.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Concentration0.8 mg/mL (after reconstitution)
      SpecificitySpecific for mouse IgG, heavy and light chain.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoaffinity Purified and Immunodepletion
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      BIOTIN CONJUGATED -2586529 2586529


      Reference overviewPub Med ID
      Expression of hepatocyte epidermal growth factor receptor, FAS and glypican 3 in EpCAM-positive regenerative clusters of hepatocytes, cholangiocytes, and progenitor cells in human liver failure.
      Hattoum, A; Rubin, E; Orr, A; Michalopoulos, GK
      Human pathology 44 743-9 2013

      Show Abstract
      23114924 23114924
      Patterns of axonal collateralization of single layer V cortical projection neurons in the rat presubiculum.
      Honda Y, Furuta T, Kaneko T, Shibata H, Sasaki H
      The Journal of comparative neurology 519 1395-412. doi 2011

      Show Abstract
      21452203 21452203
      Preventive and therapeutic potential of p38 alpha-selective mitogen-activated protein kinase inhibitor in nonobese diabetic mice with type 1 diabetes.
      Medicherla, S; Protter, AA; Ma, JY; Mangadu, R; Almirez, R; Koppelman, B; Kerr, I; Navas, TA; Movius, F; Reddy, M; Liu, YW; Luedtke, G; Perumattam, J; Mavunkel, B; Dugar, S; Schreiner, GF
      The Journal of pharmacology and experimental therapeutics 318 99-107 2006

      Show Abstract
      16603672 16603672
      Detection of Ki67 antigen by a new sheep polyclonal antiserum.
      Reynolds, GM; Rowlands, DC; Mead, GP
      Journal of clinical pathology 48 1138-40 1995

      Show Abstract Full Text Article
      8568003 8568003

      Data Sheet

      Donkey anti-Mouse IgG, Biotin Conjugated, Species Adsorbed: Hu, Rt, Bov, Eq, Gt, Sh, Rb, Chk, GP - Data Sheet

      Related Products & Applications

      Alternative Format

      Catalog Number Description  
      AP192 Donkey Anti-Mouse IgG Antibody, Species Adsorbed Show Pricing & Availability
      AP192A Donkey Anti-Mouse IgG Antibody, Alkaline Phosphatase conjugate, Species Adsorbed Show Pricing & Availability
      AP192C Donkey Anti-Mouse IgG Antibody, Cy3 conjugate, Species Adsorbed Show Pricing & Availability
      AP192F Donkey Anti-Mouse IgG Antibody, FITC conjugate, Species Adsorbed Show Pricing & Availability
      AP192P Donkey Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed Show Pricing & Availability
      AP192R Donkey Anti-Mouse IgG Antibody, Rhodamine conjugate, Species Adsorbed Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Secondary Antibodies