AP180B Donkey Anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed

AP180B
500 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP180B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDonkey Anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      References
      Product Information
      FormatBiotin
      PresentationLyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

      RECONSTITUTION:
      Reconstitute with 500 μL of sterile distilled water.
      Applications
      ApplicationDonkey anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed detects level of Donkey Goat IgG & has been published & validated for use in ELISA, FC, IF, IH & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesEIA: 1:20,000-1:400,000
      Western blots using enzyme-conjugated streptavidin: 1:20,000-1:400,000.
      Immunohistochemistry: 1:500-1:5,000
      Flow cytometry: 1:200-1:1,000
      Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000
      Optimal working dilutions must be determined by end user.
      Biological Information
      Concentration1 mg/mL
      HostDonkey
      SpecificitySpecific for Goat IgG.
      Species Reactivity
      • Goat
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoaffinity Purified and Immunodepletion
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED -28868742886874
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING -26778722677872
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY3118652
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY2886876
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY3073788
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY2901736
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY -
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - 24590452459045
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - 20263572026357
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY - 20631482063148

      References

      Reference overviewApplicationPub Med ID
      Maternal and zygotic Zfp57 modulate NOTCH signaling in cardiac development.
      Shamis, Y; Cullen, DE; Liu, L; Yang, G; Ng, SF; Xiao, L; Bell, FT; Ray, C; Takikawa, S; Moskowitz, IP; Cai, CL; Yang, X; Li, X
      Proceedings of the National Academy of Sciences of the United States of America  112  E2020-9  2015

      Show Abstract
      25848000 25848000
      Hypocretin1/orexinA-immunoreactive axons form few synaptic contacts on rat ventral tegmental area neurons that project to the medial prefrontal cortex.
      Del Cid-Pellitero, E; Garzón, M
      BMC neuroscience  15  105  2014

      Show Abstract
      25194917 25194917
      Wild-type neural progenitors divide and differentiate normally in an amyloid-rich environment.
      Yetman, MJ; Jankowsky, JL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  17335-41  2013

      Show Abstract
      24174666 24174666
      Long-term exercise is a potent trigger for ΔFosB induction in the hippocampus along the dorso-ventral axis.
      Nishijima, T; Kawakami, M; Kita, I
      PloS one  8  e81245  2013

      Show Abstract
      24282574 24282574
      Expression of hepatocyte epidermal growth factor receptor, FAS and glypican 3 in EpCAM-positive regenerative clusters of hepatocytes, cholangiocytes, and progenitor cells in human liver failure.
      Hattoum, A; Rubin, E; Orr, A; Michalopoulos, GK
      Human pathology  44  743-9  2013

      Show Abstract
      23114924 23114924
      Scaffolds containing growth factors and extracellular matrix induce hepatocyte proliferation and cell migration in normal and regenerating rat liver.
      Hammond JS, Gilbert TW, Howard D, Zaitoun A, Michalopoulos G, Shakesheff KM, Beckingham IJ, Badylak SF.
      Journal of hepatology  54  279-87  2011

      Show Abstract
      21126791 21126791
      Medial prefrontal cortex receives input from dorsal raphe nucleus neurons targeted by hypocretin1/orexinA-containing axons.
      E Del Cid-Pellitero,M Garzón
      Neuroscience  172  2011

      Show Abstract
      21036204 21036204
      Hypocretin1OrexinA-containing axons innervate locus coeruleus neurons that project to the rat medial prefrontal cortex. Implication in the sleep-wakefulness cycle and cortical activation.
      Cid-Pellitero ED, Garzón M
      Synapse  2011

      Show Abstract
      21308795 21308795
      Hypocretin1/OrexinA axon targeting of laterodorsal tegmental nucleus neurons projecting to the rat medial prefrontal cortex.
      Cid-Pellitero, Ed; Garzón, M
      Cerebral cortex (New York, N.Y. : 1991)  21  2762-73  2011

      Show Abstract
      Immunohistochemistry21508301 21508301
      Increased COX2 in the trigeminal nucleus caudalis is involved in orofacial pain induced by experimental tooth movement.
      Yuan Gao,Yin-Zhong Duan
      Anatomical record (Hoboken, N.J. : 2007)  293  2010

      Show Abstract
      20091889 20091889

      Data Sheet

      Title
      DONKEY ANTI-GOAT IgG AFFINITY PURIFIED, BIOTIN CONJUGATED ABSORBED FOR DUAL LABELING SECONDARY ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies