AP180P Donkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed

AP180P
500 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP180P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDonkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed
      Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
      References
      Product Information
      FormatHRP
      PresentationPurified donkey secondary antibody IgG in lyophilized buffer 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
      Reconstitution:
      Reconstitute with 500 μL of sterile distilled water.
      Applications
      ApplicationDonkey anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in ELISA, IH & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:500-1:5,000 dilution can be used.

      Optimal working dilutions must be determined by end user.
      Biological Information
      HostDonkey
      SpecificitySpecific for Goat IgG.
      IsotypeIgG
      Species Reactivity
      • Goat
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoaffinity Purified and Immunodepletion
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely tested on Enzyme-linked Immunosorbent Assay.

      ELISA and Western Blots:
      1:5,000-1:100,000 dilution can be used.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2-8°C for up to 12 months.
      After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
      WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb -28868772886877
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb - 20397532039753
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb - 21989282198928
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb - 31575203157520
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb - 31959713195971
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb - NG1899148NG1899148
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb - NMM1679641NMM1679641
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb -24887852488785
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb -28546562854656
      Donkey anti-Goat IgG, HRP conjugate, Species Adsorbed: H, M, R, Ch, Gp, Eq, Ht, Rb Secondary Antibody2972847

      References

      Reference overviewApplicationSpeciesPub Med ID
      Possible role of ZPAC, zygote-specific proteasome assembly chaperone, during spermatogenesis in the mouse.
      Shimizu, N; Ueno, K; Kurita, E; Shin, SW; Nishihara, T; Amano, T; Anzai, M; Kishigami, S; Kato, H; Mitani, T; Hosoi, Y; Matsumoto, K
      The Journal of reproduction and development  60  179-86  2014

      Show Abstract
      24583807 24583807
      Effects of corticosteroids in the development of limb muscle weakness in a porcine intensive care unit model.
      Aare, S; Radell, P; Eriksson, LI; Akkad, H; Chen, YW; Hoffman, EP; Larsson, L
      Physiological genomics  45  312-20  2013

      Show Abstract
      23429211 23429211
      Functional analysis of nocturnin, a circadian deadenylase, at maternal-to-zygotic transition in mice.
      Nishikawa, S; Hatanaka, Y; Tokoro, M; Shin, SW; Shimizu, N; Nishihara, T; Kato, R; Takemoto, A; Amano, T; Anzai, M; Kishigami, S; Hosoi, Y; Matsumoto, K
      The Journal of reproduction and development  59  258-65  2013

      Show Abstract
      23449310 23449310
      GSE is a maternal factor involved in active DNA demethylation in zygotes.
      Hatanaka, Y; Shimizu, N; Nishikawa, S; Tokoro, M; Shin, SW; Nishihara, T; Amano, T; Anzai, M; Kato, H; Mitani, T; Hosoi, Y; Kishigami, S; Matsumoto, K
      PloS one  8  e60205  2013

      Show Abstract
      Western Blotting23560077 23560077
      Placental melatonin production and melatonin receptor expression are altered in preeclampsia: new insights into the role of this hormone in pregnancy.
      Dave Lanoix,Pascale Guérin,Cathy Vaillancourt
      Journal of pineal research  53  2012

      Show Abstract
      22686298 22686298
      Molecular characterisation of transport mechanisms at the developing mouse blood-CSF interface: a transcriptome approach.
      Liddelow, SA; Temple, S; Møllgård, K; Gehwolf, R; Wagner, A; Bauer, H; Bauer, HC; Phoenix, TN; Dziegielewska, KM; Saunders, NR
      PloS one  7  e33554  2012

      Show Abstract
      Western Blotting, ImmunohistochemistryMouse22457777 22457777
      Protein expression and genetic structure of the coral Porites lobata in an environmentally extreme Samoan back reef: does host genotype limit phenotypic plasticity?
      Barshis DJ, Stillman JH, Gates RD, Toonen RJ, Smith LW, Birkeland C
      Molecular ecology  2010

      Show Abstract
      20345691 20345691
      Elevated temperature triggers human respiratory syncytial virus F protein six-helix bundle formation.
      Yunus, AS; Jackson, TP; Crisafi, K; Burimski, I; Kilgore, NR; Zoumplis, D; Allaway, GP; Wild, CT; Salzwedel, K
      Virology  396  226-37  2010

      Show Abstract
      19922971 19922971
      Localization of ATP-sensitive K+ channel subunits in rat submandibular gland.
      Zhou, M; He, HJ; Hirano, M; Sekiguchi, M; Tanaka, O; Kawahara, K; Abe, H
      The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society  58  499-507  2010

      Show Abstract
      19934381 19934381
      Expression and functional analyses of circadian genes in mouse oocytes and preimplantation embryos: Cry1 is involved in the meiotic process independently of circadian clock regulation.
      Amano, T; Matsushita, A; Hatanaka, Y; Watanabe, T; Oishi, K; Ishida, N; Anzai, M; Mitani, T; Kato, H; Kishigami, S; Saeki, K; Hosoi, Y; Iritani, A; Matsumoto, K
      Biology of reproduction  80  473-83  2009

      Show Abstract
      19020302 19020302

      Data Sheet

      Title
      Donkey anti-Goat IgG, Peroxidase Conjugated, Species Adsorbed: Hu, Ms, Rt, Chk, GP, Eq, Hmst, Rb - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies