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322133 | DSP, Water-Soluble

322133
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥94% by HPLC

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      322133-100MG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Glass bottle 100 mg
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          Description
          OverviewBifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups.
          Catalogue Number322133
          Brand Family Calbiochem®
          Synonyms3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
          References
          ReferencesHoward, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
          Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
          D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
          Yamada, H., et al. 1981. Biochem. 20, 4836.
          Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.
          Product Information
          FormOff-white to light tan solid
          Structure formula Image
          Applications
          Biological Information
          Purity≥94% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          322133

          References

          Reference overview
          Howard, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
          Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
          D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
          Yamada, H., et al. 1981. Biochem. 20, 4836.
          Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.

          Brochure

          Title
          Protein Modifications Tools
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-June-2008 RFH
          Synonyms3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
          DescriptionBifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups. Chemical crosslinkers are used to covalently modify proteins for studying ligand receptor interactions, conformational changes in tertiary structure, or for protein labeling. Crosslinkers are divided into homobifunctional crosslinkers, containing two identical reactive groups, or heterobifunctional crosslinkers, containing two different reactive groups. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.
          FormOff-white to light tan solid
          Structure formula
          Purity≥94% by HPLC
          SolubilityH₂O (6 mg/ml)
          Storage +2°C to +8°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
          Toxicity Irritant
          ReferencesHoward, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
          Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
          D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
          Yamada, H., et al. 1981. Biochem. 20, 4836.
          Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.