251760 DAR-4M - Calbiochem

251760
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₂₅H₂₆N₄O₃

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      251760-1MG
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          Plastic ampoule 1 mg
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          Description
          OverviewA rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).
          Catalogue Number251760
          Brand Family Calbiochem®
          SynonymsDiaminorhodamine-4M
          References
          ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
          Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
          Product Information
          ATP CompetitiveN
          DeclarationNot available for sale in Japan.
          FormRed violet solution
          FormulationIn DMSO.
          Hill FormulaC₂₅H₂₆N₄O₃
          Chemical formulaC₂₅H₂₆N₄O₃
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetNitric oxide (NO) fluorescent indicator
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 23-26-36

          Do not breathe fumes.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          251760

          References

          Reference overview
          Kojima, H., et al. 2001. Anal. Chem. 73, 1967.
          Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.

          Brochure

          Title
          Nitric Oxide and Oxidative Stress Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-September-2009 RFH
          SynonymsDiaminorhodamine-4M
          DescriptionA rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.
          FormRed violet solution
          FormulationIn DMSO.
          Intert gas (Yes/No) Packaged under inert gas
          Recommended reaction conditionsThis reagent is provided as a solution at 5 mM concentration. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol-red may affect fluorescence.
          Chemical formulaC₂₅H₂₆N₄O₃
          Structure formulaStructure formula
          Purity≥98% by HPLC
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
          Toxicity Irritant
          ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
          Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.