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251520 | DAF-FM DA - CAS 254109-22-3 - Calbiochem

251520
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₅H₁₈F₂N₂O₇ 254109-22-3

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      251520-1MG
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          Glass bottle 1 mg
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          Description
          OverviewA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
          Catalogue Number251520
          Brand Family Calbiochem®
          Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
          References
          ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
          Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
          Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
          Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
          Product Information
          CAS number254109-22-3
          ATP CompetitiveN
          DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
          FormPale yellow solution
          FormulationA 5 mM solution in DMSO.
          Hill FormulaC₂₅H₁₈F₂N₂O₇
          Chemical formulaC₂₅H₁₈F₂N₂O₇
          ReversibleN
          Structure formula Image
          Applications
          Biological Information
          Primary TargetNitric oxide (NO) fluorescent indicator
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableY
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/38

          Irritating to eyes and skin.
          S PhraseS: 26

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          251520

          References

          Reference overview
          Kojima, H., et al. 2001. J. Neurochem. 76, 1404.
          Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
          Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
          Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-April-2011 RFH
          Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
          DescriptionA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
          FormPale yellow solution
          FormulationA 5 mM solution in DMSO.
          Intert gas (Yes/No) Packaged under inert gas
          Recommended reaction conditions
          The reagent is provided at a 5 mM concentration. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol red may affect fluorescence.
          CAS number254109-22-3
          Chemical formulaC₂₅H₁₈F₂N₂O₇
          Structure formula
          Purity≥98% by HPLC
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
          Toxicity Irritant
          ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
          Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
          Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
          Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.