251500 | DAF-2 - CAS 205391-01-1 - Calbiochem

251500
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₁₄N₂O₅ 205391-01-1

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      251500-1MG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 1 mg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewA highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).
          Catalogue Number251500
          Brand Family Calbiochem®
          Synonyms4,5-Diaminofluorescein
          References
          ReferencesBerkels, R., et al. 2000. Cell Calcium 27, 281.
          Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
          Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
          Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
          Product Information
          CAS number205391-01-1
          ATP CompetitiveN
          DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
          FormPale yellow solution
          FormulationA 5 mM (1 mg in 550 µl) solution in DMSO.
          Hill FormulaC₂₀H₁₄N₂O₅
          Chemical formulaC₂₀H₁₄N₂O₅
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetFluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/38

          Irritating to eyes and skin.
          S PhraseS: 26

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          251500

          References

          Reference overview
          Berkels, R., et al. 2000. Cell Calcium 27, 281.
          Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
          Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
          Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

          Brochure

          Title
          Dyes and Stains Technical Bulletin
          Nitric Oxide and Oxidative Stress Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-April-2011 RFH
          Synonyms4,5-Diaminofluorescein
          DescriptionA highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.
          FormPale yellow solution
          FormulationA 5 mM (1 mg in 550 µl) solution in DMSO.
          Intert gas (Yes/No) Packaged under inert gas
          CAS number205391-01-1
          Chemical formulaC₂₀H₁₄N₂O₅
          Structure formulaStructure formula
          Purity≥98% by HPLC
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Irritant
          ReferencesBerkels, R., et al. 2000. Cell Calcium 27, 281.
          Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
          Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
          Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.