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ES005-100ML | DAB 50x Concentrate, Brown, Horseradish Peroxidase Substrate (insoluble in alcohol)

100 mL  
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      Key Specifications Table

      Key Applications
      WB, IHC
      Catalogue NumberES005-100ML
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDAB 50x Concentrate, Brown, Horseradish Peroxidase Substrate (insoluble in alcohol)
      OverviewSince first introduced by Graham and Karnovsky (1966), numerous procedures for its use in histochemistry, immunohistochemistry, Western and dot blots have been described (Lewis & Knight, 1977; Larsson, 1988; Gallyas et al., 1982). In the presence of horseradish peroxidase and hydrogen peroxide, DAB oxidatively polymerizes to an insoluble brown polymer. The color can be modified and intensified by treatment with metal salts of silver, copper, nickel, cobalt and osmium (Hsu & Soban, 1982). DAB is a suspected carcinogen and must be handled with caution. CHEMICON supplies DAB as a stable liquid concentrate eliminating the necessity of handling a potentially hazardous material. The stabilization system is unique and prevents formation of partially oxidized DAB excluding nonspecific binding to other heme containing proteins so often observed with powdered DAB preparations. The concentrate can be diluted in appropriate peroxide containing buffers providing the researcher with the capability of formulating any of the numerous published DAB reaction systems. The most common procedure suggests diluting the DAB 1:50 with Chemicon Catalog Number ES010 (Tris-HCl buffer, pH 7.6), and adding H(2)O(2), to a final concentration of 0.01%. Incubation times range from 5-15 minutes. Prolonged reaction time will result in non-specific staining of other heme proteins.
      Product Information
      PresentationDAB (50X) concentrate in proprietary solvents and stabilizers.
      Color CodeBlack
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for up to 12 months. DAB is a suspected carcinogen.
      Packaging Information
      Material Size100 mL
      Transport Information
      Supplemental Information



      Reference overviewPub Med ID
      Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry.
      Hsu, S M and Soban, E
      J. Histochem. Cytochem., 30: 1079-82 (1982) 1982

      Show Abstract
      6182185 6182185
      High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.
      Gallyas, F, et al.
      J. Histochem. Cytochem., 30: 183-4 (1982) 1982

      Show Abstract
      7061820 7061820
      The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
      Graham, R C and Karnovsky, M J
      J. Histochem. Cytochem., 14: 291-302 (1966) 1966

      5962951 5962951