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S8001M | CpGenome™ Human Methylated DNA Standard Set

S8001M
5 µg   
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      Overview

      Replacement Information
      Description
      Catalogue NumberS8001M
      Brand Family Upstate
      Trade Name
      • CpGenome
      • Upstate
      DescriptionCpGenome™ Human Methylated DNA Standard Set
      OverviewThe CpGenome™ Human Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. This Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase. It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome™ Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ® amplification kits.

      Materials Provided:

      One vial containing 5 µg (20 µL) of CpGenome™ Human Methylated DNA Standard at a concentration of 250 ng/µL.

      Validation:

      Methylation-specific PCR (MSP) was performed on the CpGenome™ Human Methylated DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard.

      CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
      References
      Product Information
      PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
      Applications
      ApplicationIt is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
      Application NotesFor MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Species Reactivity
      • Human
      Modifications
      • Methylation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended Storage: Stable at -20°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size5 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      CpGenome Human Methylated DNA Standard - Q2023991 Q2023991
      CpGenome™ Human Methylated -2615095 2615095
      CpGenome™ Human Methylated -2785785 2785785
      CpGenome™ Human Methylated -2808231 2808231
      CpGenome™ Human Methylated DNA Standard - 2535320 2535320
      CpGenome™ Human Methylated DNA Standard -2576401 2576401
      CpGenome™ Human Methylated DNA Standard -2702199 2702199
      CpGenome™ Human Methylated DNA Standard - 2384767 2384767
      CpGenome™ Human Methylated DNA Standard - 2468815 2468815
      CpGenome™ Human Methylated DNA Standard - 2262754 2262754

      Brochure

      Title
      Shaping Epigenetics Discovery - DNA Methylation Brochure (EMD)