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14-703M | Cot1 Protein, active, 250 µg

250 µg  Also available in 10 μg size (cat#14-703) and in bulk (cat#14-703-K).
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      Replacement Information
      Catalogue Number14-703M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCot1 Protein, active, 250 µg
      OverviewN-terminal 6His-tagged recombinant human Cot1, amino acids 30-397.
      Product Information
      ApplicationActive, N-terminal 6His-tagged recombinant human Cot1, amino acids 30-397. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceHuman Cot1, amino acids 30-397, expressed by baculovirus in Sf21 insect cells.
      Entrez Gene Number
      Entrez Gene SummaryThis gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.
      Gene Symbol
      • MAP3K8
      • TPL2
      • FLJ10486
      • C-COT
      • COT
      • c-COT
      • ESTF
      • EST
      • Tpl-2
      Protein TargetCot1
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P41279 # Able to activate NF-kappa-B 1 by stimulating proteasome- mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form of cot has some transforming activity, although it is much weaker than the activated cot oncoprotein.
      SIZE: 467 amino acids; 52925 Da
      SUBUNIT: Interacts with NFKB1/p105.
      TISSUE SPECIFICITY: Expressed in several normal tissues and human tumor-derived cell lines.
      DEVELOPMENTAL STAGE: The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle.
      PTM: Autophosphorylated. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
      SIMILARITY: SwissProt: P41279 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight45.3kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by activation of MEK1 (14-420). The activated MEK1 was used to activate MAPKinase 2/Erk2, unactive. Subsequent phosphorylation of MBP (13-104) was performed.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C from date of shipment
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-703) and in bulk (cat#14-703-K).
      Transport Information
      Supplemental Information


      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-703 Cot1 Protein, active, 10 µg Show Pricing & Availability

      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases