220477 | Chitosanase, Streptomyces sp. N174

220477
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      220477-10U
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          Plastic ampoule 10 u
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          Description
          OverviewNative Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.
          Catalogue Number220477
          Brand Family Calbiochem®
          SynonymsChitosan N-acetylglucosaminohydrolase
          References
          ReferencesBoucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
          Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
          Fukamizo, T., et al. 1995. Biochem J. 311, 377.
          Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
          Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
          Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.
          Product Information
          CAS number51570-20-8
          Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
          EC number3.2.1.132
          FormLiquid
          FormulationIn 50 M sodium acetate, 50% glycerol, pH 5.0.
          PI7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C
          Applications
          Biological Information
          Specific Activity≥25 units/mg protein
          Physicochemical Information
          Contaminantschitinolytic activity: ≤0.001%, proteases: ≤0.01%
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          220477

          References

          Reference overview
          Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
          Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
          Fukamizo, T., et al. 1995. Biochem J. 311, 377.
          Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
          Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
          Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-June-2008 RFH
          SynonymsChitosan N-acetylglucosaminohydrolase
          DescriptionNative Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.
          FormLiquid
          FormulationIn 50 M sodium acetate, 50% glycerol, pH 5.0.
          CAS number51570-20-8
          EC number3.2.1.132
          Contaminantschitinolytic activity: ≤0.001%, proteases: ≤0.01%
          Specific activity≥25 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBoucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
          Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
          Fukamizo, T., et al. 1995. Biochem J. 311, 377.
          Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
          Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
          Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.