A part of MilliporeSigma

HTS055M | ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor

200 units  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      SpeciesHost CellsProtein Target
      Human Chem-5 CXCR5
      Catalogue NumberHTS055M
      Brand Family Chemicon®
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor
      OverviewHuman CXCR5
      Background InformationCXCR5 (also known as BLR1) is a GPCR that binds selectively to the chemokine CXCL13 (also known as BLC and BCA-1) to mediate immune system development and function (Förster et al., 1996; Gunn et al., 1998). CXCR5 cooperates with CCR7 to determine functional organization of lymphoid organs such as lymph nodes and Peyer’s patches (Ohl et al., 2003). In addition, CXCR5 mediates migration of B cells into the follicles of the splenic white pulp, thus permitting antigen deposition on follicular dendritic cells (Förster et al., 1996; Cinamon et al., 2008). Patients with rheumatoid arthritis (RA) displayed upregulated expression of CXCR5 to a greater extent than other chemokine receptors, and CXCR5-null mice subjected to antigen-induced arthritis display reduced joint destruction (Schmutz et al, 2004; Wengner et al, 2007). Therefore, CXCR5 represents a potential target for treatment of rheumatoid arthritis. Millipore's CXCR5 Membrane Preparation for GTPγS Assay is a crude membrane preparation made from the proprietary stable recombinant cell line that is also used for Millipore’s CXCR5 Calcium Optimized Cell Line (HTS055C). This CXCR5 Membrane Preparation has been optimized for low basal activity in GTPγS binding assays; thus it is an ideal HTS tool for screening of compounds for agonist and antagonist activities. The membrane preparations exhibits EC50 of 1.8 nM for CXCL13 in a GTPγS binding assay.
      Product Information
      PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
      Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
      ApplicationHuman CXCR5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
      Key Applications
      • Radioligand binding assay
      • GTPγS binding
      Application NotesRadioligand binding assay and GTPγS binding
      Biological Information
      Entrez Gene Number
      Gene Symbol
      • CXCR5
      • BLR1
      • MDR15
      GPCR ClassA
      Host CellsChem-5
      Protein TargetCXCR5
      Target Sub-FamilyChemokine
      UniProt Number
      Incubation ConditionsMembranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled CXCL13 was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.

      One package contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific CXCL13-stimulated [35S]-GTPγS binding.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance1 unit = 5 μg
      EC50 in GTPγS binding assay by CXCL13: ~ 1.8 nM
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
      Packaging Information
      Material Size200 units
      Transport Information
      Supplemental Information

      Related Products & Applications

      Product Families

      Related Products By: Brand Facete


      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
      Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations