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HTSHEKT-2LT | ChemiBrite™ HEKT Parental Control 2 Frozen Cells

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      Key Specifications Table

      SpeciesHost CellsProtein Target
      Human HEKT Not Applicable
      Catalogue NumberHTSHEKT-2LT
      Trade Name
      • ChemiBrite
      DescriptionChemiBrite™ HEKT Parental Control 2 Frozen Cells
      Background InformationMillipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.

      Like Millipore’s Ready-to-Assay frozen cells, ChemiBrite frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      This product has been designed to use as a control for experiments performed with certain GPCR expressing ChemiBrite frozen cells, typically those that natively couple to Gs proteins. Millipore’s control ChemiBrite cells are made by transient transfection of HEK293T cells with optimized quantities of plasmid encoding clytin and then cryopreserving the cells at an optimal time post-transfection. These conditions closely match those that are applied to ChemiBrite frozen cells transfected with GPCRs. Upon thaw, recovery, and loading, the cells are ready for luminescent analysis to determine if the host cells respond to chemical or biological substances.
      Product Information
      • Vial contains 1 ml of mycoplasma-free cells
      ApplicationThis product has been designed to use as a control for experiments performed with certain GPCR expressing ChemiBrite frozen cells, typically those that natively couple to Gs proteins.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Host CellsHEKT
      Protein TargetNot Applicable
      Target Sub-FamilyControl
      Cell Line Type
      • Control Cell Lines
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 vial
      Transport Information
      Supplemental Information

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      Life Science Research > Drug Discovery and Development > Cell Lines for Calcium Flux Assays
      Life Science Research > Cell Culture and Systems > Cell Lines > Cell Lines for Calcium Flux Assays