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17-622 | ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set

25 assays  25 assays per kit, ~4μL per chromatin immunoprecipitation.
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, M WB, ChIP
      Catalogue Number17-622
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot,
      every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context.
      Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Trimethyl-Histone H3 (Lys27) set includes the Anti-Trimethyl-Histone H3 (Lys27) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 139 bp region of human alpha-Satellite. The Trimethyl-Histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Trimethyl-Histone H3 (Lys27)-associated chromatin.
      Alternate Names
      • H3K27me3
      • Histone H3 (tri methyl K27)
      • Histone H3K27me3
      Background InformationThe methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.
      Product Information
      • Anti-trimethyl Histone H3(Lys27) polyclonal, 1 vial
      • Polyclonal control rabbit serum, 1 vial
      • Human alpha-Satellite primer set, 1 vial
      FormatProtein A Purified
      • 1 vial containing 75μl of 5μM of each control primer specific for human alpha-Satellite. Store at -20°C.
      PresentationAnti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100 μg protein A purified IgG in 100 μL buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide. Store at -20°C.

      Normal Rabbit IgG. One vial containing 125 μg purified Rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

      ChIP Primers human alpha-Satellite. One vial containing 75 μL of 5 μM of each control primer specific for human alpha-Satellite. Store at -20°C.
      ApplicationTrimethyl-Histone H3 (Lys27) ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of H3K27Me3.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from 2x106 HeLa cells were subjected to chromatin immunoprecipitation using 4 μg purified anti-trimethyl-Histone H3 (Lys27) antibody or normal rabbit IgG and the Magna ChIP™ A kit (Cat. #17-610). Successful enrichment of trimethyl-Histone H3 (Lys27) associated DNA fragments was verified by qPCR using ChIP Primers GAPDH flanking the human GAPDH promoter and primers targeting the promoter of human MyoD.
      Please refer to the EZ-Magna ChIP™ A (Cat. #17-408) or EZChIP™ (Cat. #17-371) kit protocols for experimental details.

      Western Blot Analysis:
      Recombinant Histone H3 (Lane 2) and HeLa cell acid extracts (Lane 1) were resolved by electrophoresis, transferred to nitrocellulose and probed with a 1:5000 dilution of anti- trimethyl histone H3 Lys27 (0.4 μg/mL).
      Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
      Biological Information
      ImmunogenKLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.
      EpitopeTrimethyl Lys27
      Specificitytrimethyl-Histone H3 (Lys27)
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteBroad species cross-reactivity expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated Chromatin prepared from 3x106 NIH3T3 L1 cells were subjected to chromatin immunoprecipitation using 4 μg purified antibody or normal rabbit IgG and the Magna ChIP™ A kit (Cat. #17-610). Successful enrichment of trimethyl-Histone H3
      (Lys27) associated DNA fragments was verified by qPCR using ChIP Primers human alpha-Satellite (Please see figures).
      Please refer to the EZ-Magna ChIP™ A (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) kit protocols for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment
      Packaging Information
      Material Size25 assays
      Material Package25 assays per kit, ~4μL per chromatin immunoprecipitation.
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - 2138375 2138375
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - 2003342 2003342
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - 2069516 2069516
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - 2073749 2073749
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - 2138375A 2138375A
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - 2178413 2178413
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - 2246083 2246083
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1478803 DAM1478803
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1483223 DAM1483223
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1514145 DAM1514145
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1561713 DAM1561713
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1561715 DAM1561715
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1647850 DAM1647850
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1673215 DAM1673215
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1739345 DAM1739345
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - DAM1780446 DAM1780446
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - JBC1870087 JBC1870087
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - JBC1940033 JBC1940033
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - R0708G0054 R0708G0054
      ChIPAb+ Trimethyl-Histone H3 (Lys27) - R0708G0054 R0708G0054
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) 2465208
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) - 2488923 2488923
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) -2491143 2491143
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) -2581765 2581765
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) -2599337 2599337
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) -2604799 2604799
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) -2726786 2726786
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) -2746339 2746339
      ChIPAb+™ Trimethyl-Histone H3 -2634642 2634642
      ChIPAb+™ Trimethyl-Histone H3 -2770880 2770880
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) - 2420452 2420452
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) - 2459554 2459554
      ChIPAb+™ Trimethyl-Histone H3 (Lys27) - 2325081 2325081

      References | 65 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Histone H3 Lysine 27 demethylases Jmjd3 and Utx are required for T-cell differentiation.
      Manna, S; Kim, JK; Baugé, C; Cam, M; Zhao, Y; Shetty, J; Vacchio, MS; Castro, E; Tran, B; Tessarollo, L; Bosselut, R
      Nature communications 6 8152 2015

      Show Abstract
      26328764 26328764
      A gene expression signature identifying transient DNMT1 depletion as a causal factor of cancer-germline gene activation in melanoma.
      Cannuyer, J; Van Tongelen, A; Loriot, A; De Smet, C
      Clinical epigenetics 7 114 2015

      Show Abstract
      26504497 26504497
      Brg1 modulates enhancer activation in mesoderm lineage commitment.
      Alexander, JM; Hota, SK; He, D; Thomas, S; Ho, L; Pennacchio, LA; Bruneau, BG
      Development (Cambridge, England) 142 1418-30 2015

      Show Abstract
      25813539 25813539
      D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.
      Ma, S; Jiang, B; Deng, W; Gu, ZK; Wu, FZ; Li, T; Xia, Y; Yang, H; Ye, D; Xiong, Y; Guan, KL
      Oncotarget 6 8606-20 2015

      Show Abstract
      25825982 25825982
      In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks.
      Hu, Y; Sun, Q; Li, X; Wang, M; Cai, D; Li, X; Zhao, R
      PloS one 10 e0122643 2015

      Show Abstract
      Western Blotting25860502 25860502
      Epigenetics of Notch1 regulation in pulmonary microvascular rarefaction following extrauterine growth restriction.
      Tang, LL; Zhang, LY; Lao, LJ; Hu, QY; Gu, WZ; Fu, LC; Du, LZ
      Respiratory research 16 66 2015

      Show Abstract
      26040933 26040933
      Epigenetic memory gained by priming with osteogenic induction medium improves osteogenesis and other properties of mesenchymal stem cells.
      Rui, Y; Xu, L; Chen, R; Zhang, T; Lin, S; Hou, Y; Liu, Y; Meng, F; Liu, Z; Ni, M; Tsang, KS; Yang, F; Wang, C; Chan, HC; Jiang, X; Li, G
      Scientific reports 5 11056 2015

      Show Abstract
      26053250 26053250
      IL-21-mediated non-canonical pathway for IL-1β production in conventional dendritic cells.
      Wan, CK; Li, P; Spolski, R; Oh, J; Andraski, AB; Du, N; Yu, ZX; Dillon, CP; Green, DR; Leonard, WJ
      Nature communications 6 7988 2015

      Show Abstract
      26269257 26269257
      PIAS1 regulates breast tumorigenesis through selective epigenetic gene silencing.
      Liu, B; Tahk, S; Yee, KM; Yang, R; Yang, Y; Mackie, R; Hsu, C; Chernishof, V; O'Brien, N; Jin, Y; Fan, G; Lane, TF; Rao, J; Slamon, D; Shuai, K
      PloS one 9 e89464 2014

      Show Abstract
      24586797 24586797
      Different epigenetic alterations are associated with abnormal IGF2/Igf2 upregulation in neural tube defects.
      Bai, B; Zhang, Q; Liu, X; Miao, C; Shangguan, S; Bao, Y; Guo, J; Wang, L; Zhang, T; Li, H
      PloS one 9 e113308 2014

      Show Abstract
      25423083 25423083


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