506028 Cell Proliferation Inducer, WS3 - Calbiochem

506028
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₂₈H₃₀F₃N₇O₃•HCl

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      5.06028.0001
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          Glass bottle 10 mg
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          Description
          OverviewA cell-permeable, diarylurea analog that can reversibly induce cell proliferation of primary retinal pigment epithelial (RPE) cells from rodents and from fetal and adult human donors (~ 25 nM). Its action suggested to be mediated through Erb3 binding protein 1 (Ebp1). Non-proliferating RPE cells at passage 6 appear to respond well to WS3 treatment, whereas actively proliferating cells in passage 1 to 4 do not exhibit any increase in proliferation. These cells can differentiate into functional monolayer following WS3 withdrawal. However, WS3-expanded adult human RPE cells differentiate less effectively when compared to stem cell-derived RPE cells. The expanded RPE cells are shown to preserve vision when transplanted into rodent models of retinal degeneration. Also shown to induce proliferation of mouse R7T1 β cells (EC50 = 74 nM). Not reported to induce any genetic changes or tumorigenic events.
          Catalogue Number506028
          Brand Family Calbiochem®
          SynonymsN-(6-(4-(3-(4-((4-methylpiperazin-1-yl)methyl)-3-(trifluoromethyl)phenyl)ureido)phenoxy)pyrimidin-4-yl)cyclopropanecarboxamide Hydrochloride
          References
          ReferencesSwoboda, J.G., et al, 2013. ACS Chem. Biol. 8, 1407.
          Shen, W., et al, 2013. J. Am. Chem. Soc. 135, 1669.
          Product Information
          FormTan solid
          FormulationSupplied as a hydrochloride salt.
          Hill FormulaC₂₈H₃₀F₃N₇O₃•HCl
          Chemical formulaC₂₈H₃₀F₃N₇O₃•HCl
          ReversibleY
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥98% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Swoboda, J.G., et al, 2013. ACS Chem. Biol. 8, 1407.
          Shen, W., et al, 2013. J. Am. Chem. Soc. 135, 1669.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision18-December-2013 JSW
          SynonymsN-(6-(4-(3-(4-((4-methylpiperazin-1-yl)methyl)-3-(trifluoromethyl)phenyl)ureido)phenoxy)pyrimidin-4-yl)cyclopropanecarboxamide Hydrochloride
          DescriptionA cell-permeable, diarylurea analog that can reversibly induce cell proliferation of primary retinal pigment epithelial (RPE) cells from rodents and from fetal and adult human donors (~ 25 nM). Its action suggested to be mediated through Erb3 binding protein 1 (Ebp1). Non-proliferating RPE cells at passage 6 appear to respond well to WS3 treatment, whereas actively proliferating cells in passage 1 to 4 do not exhibit any increase in proliferation. These cells can differentiate into functional monolayer following WS3 withdrawal. However, WS3-expanded adult human RPE cells differentiate less effectively when compared to stem cell-derived RPE cells. The expanded RPE cells are shown to preserve vision when transplanted into rodent models of retinal degeneration. Also shown to induce proliferation of mouse R7T1 β cells (EC50 = 74 nM). Not reported to induce any genetic changes or tumorigenic events.
          FormTan solid
          FormulationSupplied as a hydrochloride salt.
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₂₈H₃₀F₃N₇O₃•HCl
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMSO (50 mg/ml) or H₂O (50 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesSwoboda, J.G., et al, 2013. ACS Chem. Biol. 8, 1407.
          Shen, W., et al, 2013. J. Am. Chem. Soc. 135, 1669.