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14-685 | Cdk9/Cyclin T1 Protein, active, 10 µg

14-685
10 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-685
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCdk9/Cyclin T1 Protein, active, 10 µg
      OverviewC-terminal 6His-tagged, fulllength recombinant, human cdk9 co-expressed with untagged, full-length human cyclin T1
      References
      Product Information
      Applications
      ApplicationActive, C-terminal 6His-tagged, full length recombinant, human Cdk9 co-expressed with untagged, full-length human Cyclin T1, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
      Gene Symbol
      • CDK9
      • C-2k
      • PITALRE
      • TAK
      • CDC2L4
      • C-2K
      Protein TargetCDK9/cyclin T1
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P50750 # Member of the cyclin-dependent kinase pair (CDK9/cyclin- T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
      SIZE: 372 amino acids; 42778 Da
      SUBUNIT: Associates with CCNT1/cyclin-T1 to form P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Also associates with CCNK/cyclin-K. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous.
      SIMILARITY: SwissProt: P50750 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
      Molecular Weight44kDa (cdk9) and 80.79kDa (cyclinT1)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of PDKtide (Catalog# 12-401)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditionsstable for 6 months at -70°C from date of shipment
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Cdk9/cyclin T1, active - 2021338-B 2021338-B
      Cdk9/cyclin T1, active - 2115348-B 2115348-B
      Cdk9/cyclin T1, active - D8KN087U-K D8KN087U-K
      Cdk9/cyclin T1, active - D8KN087U-M D8KN087U-M
      Cdk9/cyclin T1, active - D8KN087U-N D8KN087U-N
      Cdk9/cyclin T1,active - 2115348-C 2115348-C
      Cdk9′cyclin T1,active - 2115348-C

      References

      Reference overviewPub Med ID
      HIV-1 infection and regulation of Tat function in macrophages.
      Liou, Li-Ying, et al.
      Int. J. Biochem. Cell Biol., 36: 1767-75 (2004) 2004

      Show Abstract
      15183343 15183343
      Cellular control of gene expression by T-type cyclin/CDK9 complexes.
      Garriga, Judit and Graña, Xavier
      Gene, 337: 15-23 (2004) 2004

      Show Abstract
      15276198 15276198
      Regulation of TAK/P-TEFb in CD4+ T lymphocytes and macrophages.
      Rice, Andrew P and Herrmann, Christine H
      Curr. HIV Res., 1: 395-404 (2003) 2003

      Show Abstract
      15049426 15049426
      Cyclins that don't cycle--cyclin T/cyclin-dependent kinase-9 determines cardiac muscle cell size.
      Sano, Motoaki and Schneider, Michael D
      Cell Cycle, 2: 99-104 (2003) 2003

      Show Abstract
      12695656 12695656

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      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases