17-286 Cdc42 Activation Assay Kit

17-286
1 kit  Kit capacity: 30 assays
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsDetection Methods
      ABA Chemiluminescence
      Description
      Catalogue Number17-286
      ReplacesSGT430
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCdc42 Activation Assay Kit
      References
      Product Information
      Components
      • Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
      • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      • Anti-cdc42
      Detection methodChemiluminescence
      Applications
      ApplicationNon-radioactive cdc42 Activation Assay Kit can be used to precipitate cdc42-GTP from cell lysates subjected to various treatments & detection by a cdc42 specific monoclonal antibody.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
      Gene Symbol
      • CDC42
      • G25K
      • CDC42Hs
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P60953 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
      SIZE: 191 amino acids; 21311 Da
      SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
      SIMILARITY: SwissProt: P60953 ## Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoprecipitating cdc42-GTP from 3T3/NIH cell lysate that has been loaded in vitro with GTPγS. Subsequent detection in western immunoblot using anti-cdc42.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 30 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      cdc42 Activation Assay Kit (non-radioactive) - 21510692151069
      cdc42 Activation Assay Kit (non-radioactive) - 23904952390495
      cdc42 Activation Assay Kit (non-radioactive) - 24532602453260
      cdc42 Activation Assay Kit (non-radioactive)2915453
      cdc42 Activation Assay Kit (non-radioactive)2989599
      cdc42 Activation Assay Kit (non-radioactive) - 21096712109671
      cdc42 Activation Assay Kit (non-radioactive) - 21975222197522
      cdc42 Activation Assay Kit (non-radioactive) - 22460732246073
      cdc42 Activation Assay Kit (non-radioactive) - DAM1411264DAM1411264
      cdc42 Activation Assay Kit - 06110445530611044553

      References

      Reference overviewApplicationPub Med ID
      Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements.
      Zhi-Jie Cheng, Raman Deep Singh, Deepak K Sharma, Eileen L Holicky, Kentaro Hanada, David L Marks, Richard E Pagano
      Molecular biology of the cell  17  3197-210  2006

      Show Abstract Full Text Article
      16672382 16672382
      Cdc42 is a Rho GTPase family member that can mediate insulin signaling to glucose transport in 3T3-L1 adipocytes
      Usui, I., et al
      J Biol Chem, 278:13765-74 (2003)  2003

      Activation Assay12566459 12566459
      Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases.
      Benard, V, et al.
      J. Biol. Chem., 274: 13198-204 (1999)  1999

      10224076 10224076
      Cell cycle-dependent activation of Ras.
      Taylor, S J and Shalloway, D
      Curr. Biol., 6: 1621-7 (1996)  1996

      Show Abstract
      8994826 8994826

      Brochure

      Title
      Hallmarks of Aging (EMD)

      Related Products & Applications

      Kit Component

      Catalog Number Description  
      14-325 Rac/cdc42 Assay Reagent (PAK1 PBD, agarose), 300 µg Show Pricing & Availability
      20-168 Mg2+ Lysis/Wash Buffer, 5X Show Pricing & Availability
      20-176 100X GTPγS, 10mM Show Pricing & Availability
      20-177 100X GDP, 100mM Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays