219360 | Cathepsin D/E Substrate, Fluorogenic - Calbiochem

219360
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      219360-1MG
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          Plastic ampoule 1 mg
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          Description
          OverviewAn internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
          Catalogue Number219360
          Brand Family Calbiochem®
          SynonymsMOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
          References
          ReferencesYasuda, Y., et al. 1999. J. Biochem. 126, 260.
          Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.
          Product Information
          ATP CompetitiveN
          FormYellow lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₈₅H₁₂₂N₂₂O₁₉
          Chemical formulaC₈₅H₁₂₂N₂₂O₁₉
          Hygroscopic Hygroscopic
          ReversibleN
          Applications
          ApplicationCathepsin D/E Substrate, Fluorogenic, is an internally quenched fluorogenic 11-mer peptide that acts as a selective substrate for cathepsins D and E.
          Biological Information
          Primary TargetSubstrate for cathepsins D and E
          Primary Target IC<sub>50</sub>kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively
          Purity≥97% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Peptide SequenceMOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂
          MOCAc = (7-Methoxycoumarin-4-yl)acetyl
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          219360

          References

          Reference overview
          Yasuda, Y., et al. 1999. J. Biochem. 126, 260.
          Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-June-2008 RFH
          SynonymsMOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
          DescriptionAn internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.
          FormYellow lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Intert gas (Yes/No) Packaged under inert gas
          Recommended reaction conditions80 µl of 50 mM sodium acetate buffer, pH 4.0, 10 µl of 200 µM substrate solution, 10 µl of sample, incubate at 40°C for 10 min; terminate reaction with 2 ml of 5% TCA.
          Chemical formulaC₈₅H₁₂₂N₂₂O₁₉
          Peptide SequenceMOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂
          MOCAc = (7-Methoxycoumarin-4-yl)acetyl
          Purity≥97% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage +2°C to +8°C
          Hygroscopic
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesYasuda, Y., et al. 1999. J. Biochem. 126, 260.
          Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.