Key Specifications Table
|Key Applications||Entrez Gene Number||Species||Uni Prot Number|
|ACT||NM_001080124.1, NM_001080125.1, NM_001228.4, NM_033355.3, NM_033356.3, NM_033358.3||Human||Q14790|
|Presentation||Lyophilized. Reconstitute with 25 μl PBS containing 15% glycerol.|
|Application Notes||Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays.
Optimal working dilutions must be determined by end user.
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at -70°C for 3-6 months. Avoid repeated freeze/thaw cycles.|
|Material Size||25 units|
|Reference overview||Pub Med ID|
|High molecular weight vimentin complex is formed after proteolytic digestion of vimentin by caspase-3: detection by sera of patients with interstitial pneumonia. |
Fujita, Jiro, et al.
Microbiol. Immunol., 47: 447-51 (2003) 2003
In a previous study, we demonstrated anti-vimentin antibodies in sera of patients with interstitial pneumonia. We hypothesized that antibodies in sera might detect vimentin fragments formed during the process of apoptosis. To prove this, recombinant human vimentin was digested by recombinant human caspase-3 or caspase-8. Then, Western blotting using several commercially available antibodies against human vimentin or patients' sera which had anti-vimentin autoantibodies, was performed. As a result, after recombinant human vimentin was digested by caspase-3 or caspase-8, several vimentin fragments were formed and detected by 2 kinds of monoclonal anti-vimentin antibodies (clone 3B4 and clone V9) as well as by polyclonal sheep anti-human vimentin antibody. It was demonstrated that high molecular weight vimentin was formed after the digestion of vimentin by caspase-3, which was only detected by patients' sera. The high molecular weight vimentin was not formed after digestion of vimentin by caspase-8. Our present results show that high molecular weight vimentin was formed after the digestion of vimentin by caspase-3. In addition, it is suggested that this high molecular weight vimentin acted as an autoantigen to form anti-vimentin autoantibody in vivo.
|Specific caspase pathways are activated in the two stages of cerebral infarction. |
A Benchoua,C Guégan,C Couriaud,H Hosseini,N Sampaïo,D Morin,B Onténiente
The Journal of neuroscience : the official journal of the Society for Neuroscience 21 2001
Necrosis and apoptosis have been initially identified as two exclusive pathways for cell death. In acute brain lesions, such as focal ischemia, this binary scheme is challenged by demonstrations of mixed morphological and biochemical characteristics of both apoptosis and necrosis in single cells. The resulting difficulty in defining the nature of cell death that is triggered by severe insults has dramatically impeded the development of therapeutic strategies. We show that in the early stages of cerebral infarction, neurons of the so-called necrotic core display a number of morphological, physiological, and biochemical features of early apoptosis, which include cytoplasmic and nuclear condensations and specific caspase activation cascades. Early activation cascades involve the death receptor pathway linked to caspase-8 and the caspase-1 pathway. They are not associated with alterations of mitochondrial respiration or activation of caspase-9. In contrast, pathways that are activated during the secondary expansion of the lesion in the penumbral area include caspase-9. In agreement with its downstream position in both mitochondria-dependent and -independent pathways, activation of caspase-3 displays a biphasic time course. We suggest that apoptosis is the first commitment to death after acute cerebral ischemia and that the final morphological features observed results from abortion of the process because of severe energy depletion in the core. In contrast, energy-dependent caspase activation cascades are observed in the penumbra in which apoptosis can fully develop because of residual blood supply.
|Caspase 8, recombinant human active - Data Sheet|