A part of MilliporeSigma

QIA70 | Caspase-3 Activity Assay Kit, Fluorometric

QIA70
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityDetection Methods
      A Broad Range Of Species Fluorometric

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      QIA70-1KIT
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 1 kit
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewSuitable for the in vitro detection of caspase-3 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Nucleosome ELISA Kit (Cat. No. QIA25) and the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20). A high-throughput drug-screening version is also available (Cat. No. HTS02).
          Catalogue NumberQIA70
          Brand Family Calbiochem®
          Materials Required but Not Delivered 1 M dithiothreitol (DTT, Cleland regent) solution (1.54 g to 10 ml H2O) made fresh (can be stored at -20°C for one month).
          2-20 µl, 20-200 µl and 200-1000 µl precision pipettes with disposable tips.
          Fluorescent plate reader capable of measuring excitation ~400 nm, and emission at ~505 nm.
          A centrifuge, for spinning tubes or 96-well plate and to pulse-spin the Recombinant Caspase-3 vials.
          Microcentrifuge tubes.
          Protease inhibitor cocktail set III (Cat. No. 539134)

          Note: It is recommended to add protease inhibitor cocktail at a 1:500 dilution to Sample Buffer and Assay Buffer just before use. These protease inhibitors have been shown to exhibit no effect on recombinant caspase-3 activity.
          References
          ReferencesX. Sun, et al. 1999. J. Biol. Chem. 274, 5053.
          N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312.
          S. J. Martin and D. 1995 R. Green, Cell 82, 349.
          Product Information
          Detection methodFluorometric
          Form96 Tests
          Format96-well plate
          Kit contains96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol.
          Applications
          Biological Information
          Assay time2.5-3 h
          Sample TypeCell lysates
          Species Reactivity
          • A Broad Range Of Species
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36-23

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Do not breathe fumes.
          Product Usage Statements
          Intended useThe Calbiochem Caspase-3 Activity Assay is a non-isotopic enzyme assay for the in vitro detection of Caspase-3 activity in cell lysates. The assay can be adapted for 96-well plates for sensitive drug screening methods.
          Storage and Shipping Information
          Ship Code Multiple Storage Conditions
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage Multiple storage requirements
          Storage ConditionsUpon Receipt of the kit: Store Recombinant Caspase-3 (2 vials) at -80°C to maintain stability. Store all other kit components at -20°C. Once thawed, Caspase-3 Sample Buffer and Assay Buffer may be stored at 4°C. Do not expose reagents to excessive light.
          Protect from Moisture Protect from moisture
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Kit contains96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol.
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          QIA70

          References

          Reference overview
          X. Sun, et al. 1999. J. Biol. Chem. 274, 5053.
          N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312.
          S. J. Martin and D. 1995 R. Green, Cell 82, 349.

          Citations

          Title
        • So-Young Park and Adriana Ferreira. (2005) The generation of a 17 kDa neurotoxic fragment: an alternative mechanism by which Tau mediates β-amyloid-induced neurodegeneration. Journal of Neuroscience 25, 5365-5375.