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218704 | Casein Kinase II, α-Subunit, Human, Recombinant, E. coli

218704
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      218704-10MIU
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 10 miu
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          Description
          OverviewRecombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
          Note: 1 mU = 1 milliunit.
          Catalogue Number218704
          Brand Family Calbiochem®
          SynonymsrhCKIIα, Protein Kinase CKIIα
          References
          ReferencesAllende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
          Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
          Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
          Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.
          FormLiquid
          FormulationIn 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.
          Applications
          Biological Information
          Purity≥95% by SDS-PAGE
          Specific Activity≥1.0 unit/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Contaminantsslight autoproteolysis yields one additional band of 39 kDa; however, no loss in activity is associated with the appearance of this 39 kDa product
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Certificates of Analysis

          TitleLot Number
          218704

          References

          Reference overview
          Allende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
          Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
          Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
          Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision21-May-2008 JSW
          SynonymsrhCKIIα, Protein Kinase CKIIα
          DescriptionRecombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where the autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt sensitive with respect to its activity. Easily inactivated by treatment with heat, trypsin digestion, or urea.
          FormLiquid
          FormulationIn 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥95% by SDS-PAGE
          Contaminantsslight autoproteolysis yields one additional band of 39 kDa; however, no loss in activity is associated with the appearance of this 39 kDa product
          Specific activity≥1.0 unit/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesAllende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
          Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
          Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
          Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.