208773 | Calpain Substrate IV, Cell-Permeable, Fluorogenic - Calbiochem

208773
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      208773-1MG
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          Description
          OverviewThe non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
          Catalogue Number208773
          Brand Family Calbiochem®
          Synonyms(DABCYL)-TPLK~SPPPSPRE(EDANS)-RRRRRRR-NH₂
          References
          ReferencesBanoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.
          Product Information
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt
          Hill FormulaC₁₂₇H₂₀₆N₅₀O₂₇S
          Chemical formulaC₁₂₇H₂₀₆N₅₀O₂₇S
          Hygroscopic Hygroscopic
          Applications
          Biological Information
          Purity≥95% by HPLC
          Physicochemical Information
          Emission max.
          Excitation max.
          Peptide Sequence(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          208773

          References

          Reference overview
          Banoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision23-July-2014 JSW
          Synonyms(DABCYL)-TPLK~SPPPSPRE(EDANS)-RRRRRRR-NH₂
          DescriptionThe non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₂₇H₂₀₆N₅₀O₂₇S
          Peptide Sequence(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂
          Purity≥95% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage -20°C
          Hygroscopic
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesBanoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.