208706 | Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda

208706
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      208706-5000U
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 5000 u
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          Description
          OverviewRecombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. Reportedly involved in the modulation of neurite outgrowth by cAMP. Lower levels of CaM kinase II α-subunit predispose neurons to ischemic damage.
          Catalogue Number208706
          Brand Family Calbiochem®
          SynonymsCaM Kinase II
          References
          ReferencesTashima, K., et al. 1996. J. Neurochem. 66, 57.
          Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
          Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
          Product Information
          Activity≥250,000 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Physicochemical Information
          ContaminantsPhosphatases, proteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          208706

          References

          Reference overview
          Tashima, K., et al. 1996. J. Neurochem. 66, 57.
          Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
          Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.

          Brochure

          Title
          Calmodulin and Related Products Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-May-2008 JSW
          SynonymsCaM Kinase II
          DescriptionRecombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.
          Recommended reaction conditions1. Dilute CaM kinase II into 20 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT, and 0.1 mM EDTA, pH 7.5.2. 2. Add 2.4 μM calmodulin, 2 mM CaCl2, 100 µM ATP, and [32P]-γATP at 100 µCi/µmole 3. Incubate at 30°C.
          Purity≥90% by SDS-PAGE
          ContaminantsPhosphatases, proteases: none detected
          Activity≥250,000 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesTashima, K., et al. 1996. J. Neurochem. 66, 57.
          Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
          Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.