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14-618M | CD45 Protein, 250 µg

14-618M
250 µg  Also available in 10 μg size (cat#14-618) and in bulk (cat#14-618-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-618M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCD45 Protein, 250 µg
      OverviewHis6-tagged, recombinant, human CD45, amino acids 598-end
      References
      Product Information
      Applications
      ApplicationHis6-tagged, recombinant, human CD45, amino acids 598-end, for use in Enzyme Assays.
      Key Applications
      • Enzyme Assay
      Biological Information
      Sourceexpressed in E. coli
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
      Gene Symbol
      • PTPRC
      • CD45
      • LY5
      • T200
      • glycoprotein
      • L-CA
      • LCA
      • B220
      • GP180
      Protein TargetCD45
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTyr phosphatase
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
      SIZE: 1304 amino acids; 147254 Da
      SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
      PTM: Heavily N- and O-glycosylated.
      DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
      SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
      Molecular Weight84kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by dephosphorylation of 100μM DiFMUP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-618) and in bulk (cat#14-618-K).
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-618 CD45 Protein, 10 µg Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Phosphatases
      Life Science Research > Proteins and Enzymes > Purified Phosphatases