GF101 | CD40 Ligand/TRAP Protein, Recombinant human

GF101
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT Human Recombinant human Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.
      Description
      Catalogue NumberGF101
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCD40 Ligand/TRAP Protein, Recombinant human
      OverviewCD40 Ligand (CD40L/TRAP) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells. GF101 is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
      Alternate Names
      • CD154
      • TRAP
      • TNF-related activation protein
      • T cell antigen Gp39
      References
      Product Information
      PresentationSterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5.
      Applications
      ApplicationThe CD40 Ligand (CD40L/TRAP protein) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells.
      Key Applications
      • Cell Culture
      Biological Information
      PurityGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.
      SourceRecombinant human
      Specific ActivityGF101 is fully biologically active as determined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PMBC). It also stimulates IL-8 induction by human PBMC using a concentration of 5-10 ng/mL.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
      Gene Symbol
      • CD40LG
      • CD154
      • TRAP
      • gp39
      • CD40L
      • IMD3
      • IGM
      • hCD40L
      • TNFSF5
      • HIGM1
      • CD40-L
      • T-BAM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29965 # Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      SIZE: 261 amino acids; 29274 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & CD40 ligand, soluble form: Secreted.
      TISSUE SPECIFICITY: Specifically expressed on activated CD4+ T- lymphocytes.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. & Not O-glycosylated.
      DISEASE: SwissProt: P29965 # Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
      SIMILARITY: SwissProt: P29965 ## Belongs to the tumor necrosis factor family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in 10 mM Sodium Phosphate, pH7.5 to a concentration of 0.1-1 mg/mL. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Recombinant Human soluble CD40 Ligand / TRAP - 2119983 2119983
      Recombinant Human soluble CD40 Ligand / TRAP - 2105104 2105104
      Recombinant Human soluble CD40 Ligand / TRAP - 2297175 2297175
      Recombinant Human soluble CD40 Ligand / TRAP -2651984 2651984
      Recombinant Human soluble CD40 Ligand / TRAP -2653139 2653139
      Recombinant Human soluble CD40 Ligand / TRAP -2661376 2661376
      Recombinant Human soluble CD40 Ligand / TRAP -2707836 2707836
      Recombinant Human soluble CD40 Ligand / TRAP -2775712 2775712
      Recombinant Human soluble CD40 Ligand / TRAP -2785825 2785825
      Recombinant Human soluble CD40 Ligand / TRAP CATALOG - 2203060 2203060

      Data Sheet

      Title
      Recombinant Human soluble CD40 Ligand / TRAP

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors