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14-575 | CARM1, active protein, N-terminal His-tagged

10 µg  
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      Replacement Information

      Key Specifications Table

      Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
      HMT NM_199141.1 Mouse Q86X55
      Catalogue Number14-575
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCARM1, active protein, N-terminal His-tagged
      OverviewN-terminal His-tagged, recombinant mouse CARM1, expressed in Sf9 cells. Purified using Ni-NTA agarose chromatography
      Product Information
      ApplicationN-terminal His-tagged, recombinant mouse CARM1, expressed in Sf9 cells. Purified using Ni-NTA agarose chromatography.
      Key Applications
      • Histone Methyltransferase Assay (HMT)
      Biological Information
      PurityGreater than 75% by SDS-PAGE.
      SourceRecombinant mouse CARM expressed in Sf9 cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryProtein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM]
      Gene Symbol
      • CARM1
      • PRMT4
      Purification MethodNi-NTA agarose chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q86X55 # Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.

      SIZE: 585 amino acids; 63460 Da

      SUBUNIT: Homodimer (Probable). Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, CREBBP and CTNNB1. Interacts with SNRPC (By similarity). Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm.

      TISSUE SPECIFICITY: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

      SIMILARITY: SwissProt: Q86X55 ## Belongs to the protein arginine N-methyltransferase family.

      MISCELLANEOUS: Methylation of H3-R17 by CARM1 is stimulated by preacetylation of H3-K18/H3-K23 by EP300 and blocked by citrullination of H3-R17 by PADI4 (By similarity).
      Molecular Weight~68 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by methylalization of histone H3
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      CARM1, active (recombinant protein expressed in Sf9) - DAM1473541 DAM1473541
      CARM1, active - 26792 26792
      CARM1, active - R0706D0004 R0706D0004


      Reference overviewPub Med ID
      Regulation of transcription by a protein methyltransferase.
      Chen, D, et al.
      Science, 284: 2174-7 (1999) 1999

      Show Abstract
      10381882 10381882

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      Life Science Research > Proteins and Enzymes > Other Enzymes