Key Specifications Table
|Key Applications||Entrez Gene Number||Species||Uni Prot Number|
|Application||N-terminal His-tagged, recombinant mouse CARM1, expressed in Sf9 cells. Purified using Ni-NTA agarose chromatography.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||6 months at -70°C.|
|Material Size||10 µg|
|CARM1, active (recombinant protein expressed in Sf9) - DAM1473541||DAM1473541|
|CARM1, active - 26792||26792|
|CARM1, active - R0706D0004||R0706D0004|
|Reference overview||Pub Med ID|
|Regulation of transcription by a protein methyltransferase. |
Chen, D, et al.
Science, 284: 2174-7 (1999) 1999
The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors, but only when GRIP1 or SRC-1a was coexpressed. Thus, CARM1 functions as a secondary coactivator through its association with p160 coactivators. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduced both methyltransferase and coactivator activities. Thus, coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.