2750 BrdU Cell Proliferation Kit

2750
200 assays  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsDetection Methods
      ELISA Colorimetric
      Description
      Catalogue Number2750
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionBrdU Cell Proliferation Kit
      OverviewTest Principle

      With CHEMICON®'s BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.
      Materials Required but Not Delivered1. Pipettors & tips capable of accurately measuring 2-1000 μL

      2. Multi-channel or repeating pipettes

      3. Wash bottle or multichannel dispensor for washing

      4. 2000 mL graduated cylinder

      5. PBS (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4-7H2O, 1.4 mM KH2PO4)

      6. Deionized or distilled H2O.

      7. 96-well microplate reader with 450-540 nm or 450-595 nm dual wavelength or 450 nm single wavelength filter

      8. Tissue culture microplate (96 well culture dish)

      9. Sterile reagent troughs

      10. Micro syringe filter (0.2 μm)

      11. Syringe
      Background InformationEvaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has been a traditional method for detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting of autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.

      A well established alternative to [3H] thymidine uptake has been demonstrated by numerous researchers. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are synthesizing DNA.
      References
      Product Information
      Components
      • BrdU Reagent: (Part No. 2750a) One 15 μL vial of a 500X solution of BrdU.
      • Fixing Solution: (Part No. 2750b) One 40 mL bottle.
      • Prediluted BrdU Detection Antibody: (Part No. 2750c) One 20 mL vial of an anti-BrdU solution.
      • Stop Solution: (Part No. 2750d): One 25 mL bottle of 2.5N sulfuric acid solution.
      • Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2750e) One 15 μL vial of a 2000X solution.
      • Conjugate Diluent: (Part No. 2750f) One 25 mL bottle.
      • Substrate: (Part No. 2750g) One 25 mL bottle, ready to use TMB.
      • 50X Plate Wash Concentrate: (Part No. 2750h) One 90 mL bottle of buffered Tris with surfactant.
      Detection methodColorimetric
      Applications
      ApplicationThe BrdU Cell Proliferation Assay Kit is used to detect the BrdU in a sample.
      Key Applications
      • ELISA
      Application NotesThe CHEMICON® BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells.

      For Research Use Only. Not for use in diagnostic procedures.
      Biological Information
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Pig
      • Horse
      • Rabbit
      • Guinea Pig
      • Hamster
      • Non-human Primate
      • Canine
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain the kit at 2° to 8°C under sterile conditions for up to 6 months after date of receipt.

      Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution.
      Packaging Information
      Material Size200 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      BrdU Cell Proliferation Kit SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      A fluorescence polarization based screening assay for identification of small molecule inhibitors of the PICK1 PDZ domain.
      Thor S Thorsen,Kenneth L Madsen,Tino Dyhring,Anders Bach,Dan Peters,Kristian Strømgaard,Lars Christian B Rønn,Ulrik Gether
      Combinatorial chemistry & high throughput screening  14  2011

      Show Abstract
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      The immunoproteasome, the 20S proteasome, and the PA28αβ proteasome regulator are oxidative stress-adaptive proteolytic complexes.
      Pickering AM, Koop AL, Teoh CY, Ermak G, Grune T, Davies KJ
      Biochem J  2010

      Show Abstract
      20919990 20919990
      Isatis tinctoria L. combined with co-stimulatory molecules blockade prolongs survival of cardiac allografts in alloantigen-primed mice.
      Kang X, Chen J, Qin Q, Wang F, Wang Y, Lan T, Xu S, Wang F, Xia J, Ekberg H, Qi Z, Liu Z
      Transpl Immunol  2010

      Show Abstract
      20338239 20338239
      Induction of dopaminergic neurons from growth factor expanded neural stem/progenitor cell cultures derived from human first trimester forebrain.
      Nicolaj S Christophersen,Xia Meijer,Jesper R Jørgensen,Ulrica Englund,Mette Grønborg,Ake Seiger,Patrik Brundin,Lars U Wahlberg
      Brain research bulletin  70  2006

      Show Abstract
      17027782 17027782
      Gene marking of human neural stem/precursor cells using green fluorescent proteins.
      Beatriz Navarro-Galve,Ana Villa,Carlos Bueno,Lachlan Thompson,Jens Johansen,Alberto Martínez-Serrano
      The journal of gene medicine  7  2005

      Show Abstract
      15508144 15508144
      Application of triple-probe microdialysis for fast pharmacokinetic/pharmacodynamic evaluation of dopamimetic activity of drug candidates in the rat brain.
      Pia Weikop,Börje Egestad,Jan Kehr
      Journal of neuroscience methods  140  2004

      Show Abstract
      15589335 15589335
      Plague in San Francisco: an essay review.
      Robert Barde
      Journal of the history of medicine and allied sciences  59  2004

      15270338 15270338
      Effect of mutant alpha-synuclein on dopamine homeostasis in a new human mesencephalic cell line.
      Julie Lotharius,Sebastian Barg,Pia Wiekop,Cecilia Lundberg,Heather K Raymon,Patrik Brundin
      The Journal of biological chemistry  277  2002

      Show Abstract
      12145295 12145295
      Designing new metal affinity peptides by random mutagenesis of a natural metal-binding site.
      M M Enzelberger, S Minning, R D Schmid
      Journal of chromatography. A  898  83-94  2000

      Show Abstract
      11185626 11185626
      Novel HY peptide antigens presented by HLA-B27.
      W A Simmons,S G Summerfield,D C Roopenian,C A Slaughter,A R Zuberi,S J Gaskell,R S Bordoli,J Hoyes,C R Moomaw,R A Colbert,L Y Leong,G W Butcher,R E Hammer,J D Taurog
      Journal of immunology (Baltimore, Md. : 1950)  159  1997

      Show Abstract
      9300696 9300696

      Technical Info

      Title
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions

      User Guides

      Title
      BrdU Cell Proliferation Assay 200 Tests