382065 Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem

382065
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₇H₂₈N₆O · 3HCl 23491-52-3

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      382065-100MG
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          Alu drum 100 mg
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          Description
          OverviewCell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.
          Catalogue Number382065
          Brand Family Calbiochem®
          Synonyms2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
          References
          ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
          Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
          Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
          Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
          Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
          Sandhu, L.C., et al. 1985. Cytometry 6, 191.
          Frankfurt, O.S. 1983. Cytometry 4, 216.
          Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
          Visser, J.W.M. 1980. Flow Cytometry IV, 86.
          Product Information
          CAS number23491-52-3
          ATP CompetitiveN
          FormYellow solid
          Hill FormulaC₂₇H₂₈N₆O · 3HCl
          Chemical formulaC₂₇H₂₈N₆O · 3HCl
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetAdenine-thymine-specific fluorescent stain
          Purity≥95% by TLC
          Physicochemical Information
          Cell permeableY
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSDT4200000
          Safety Information
          R PhraseR: 23/24/25-36/37/38

          Toxic by inhalation, in contact with skin and if swallowed.
          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36/37/39-45

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Toxic & Carcinogenic / Teratogenic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          382065

          References

          Reference overview
          Werstuck, G., and Green, M.R. 1998. Science 282, 296.
          Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
          Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
          Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
          Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
          Sandhu, L.C., et al. 1985. Cytometry 6, 191.
          Frankfurt, O.S. 1983. Cytometry 4, 216.
          Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
          Visser, J.W.M. 1980. Flow Cytometry IV, 86.

          Brochure

          Title
          Dyes and Stains Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-July-2008 RFH
          Synonyms2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
          DescriptionCell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.
          FormYellow solid
          CAS number23491-52-3
          RTECSDT4200000
          Chemical formulaC₂₇H₂₈N₆O · 3HCl
          Structure formulaStructure formula
          Purity≥95% by TLC
          SolubilityH₂O (50 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
          Toxicity Toxic & Carcinogenic / Teratogenic
          ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
          Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
          Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
          Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
          Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
          Sandhu, L.C., et al. 1985. Cytometry 6, 191.
          Frankfurt, O.S. 1983. Cytometry 4, 216.
          Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
          Visser, J.W.M. 1980. Flow Cytometry IV, 86.

          Related Products & Applications

          Alternative Products

          Catalog Number Description  
          382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem Show Pricing & Availability

          Categories

          Life Science Research > Inhibitors and Biochemicals > Biochemicals > Dyes, Stains and Probes > DNA-Binding Dyes