L6155-BC Biocoll, Density 1.10 g/ml

L6155-BC
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      L6155
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          500 ml
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          Description
          OverviewBiocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. Densities of up to 1.1 g/ml can be adjusted using this hydrophilic polymer. For optimal pH and osmolality, adjusting Biocoll with an acid, preferably amidotrizoeic acid(ATA), and sodium hydroxide is required. Biocoll with densities of 1.077 g/ml (cat. no. L6113/5) and 1.090 g/ml (cat. no. L6125) are already adjusted accordingly.

          Separating solutions
          Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

          Recommendations:
          The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value. But you can also adjust a required density yourself diluting Easycoll correspondingly.
          Table 36: Instructions on how to dilute the stock solution of Biocoll (density 1.090 g/ml; cat. no. L6125) with PBS (cat. no. L1825/0)
          All amounts given for a temperature of +20°C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions will influence agglomeration and increase formation of cell clusters.

          Separating lymphocytes using Biocoll
          1. Biocoll separating solution (D = 1.077 at +20°C) is given to 15 or 25 ml centrifugal tubes in volumes of 7 ml and 10 ml, resp.
          2. Equal parts of heparinized whole blood (50 U/ml heparin, stabilizer-free) and culture medium are mixed and carefully applied over Biocoll separating solution.
          3. Centrifuge 1200xg for 20 min.
          4. Take layer of enriched (70 - 100%) lymphocytes (between plasma and Biocoll) with a Pasteur′s pipette and wash twice in culture medium:
          • for 10 min at 300xg
          • for 10 min at 200xg
          5. Cell count as usual.
          Specifications:

          Sterility: Pass
          pH: 6.6-7.5
          Osmolality: 0.325-0.450 Osm/kg H20
          Density: 1.099-1.101 g/ml
          Color: Colorless
          Appearance: Clear

          Catalogue NumberL6155-BC
          Brand Family Calbiochem®
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          Ship Code Ambient Temperature Only
          Storage +2°C to +25°C
          Do not freeze Ok to freeze
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