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70664 | Benzonase® Nuclease, Purity > 99%

70664
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      Overview

      Replacement Information

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      70664-3
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          Glass bottle 10 ku
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          Description
          OverviewBenzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.

          The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.

          Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied in 50% glycerol. Store at -20°C.

          Total endotoxin: < 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
          Catalogue Number70664
          Brand Family Novagen®
          References
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
          Applications
          Biological Information
          Purity> 99% by SDS-PAGE
          Physicochemical Information
          ContaminantsTotal endotoxin: < 0.25 EU/1000 units.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          70664

          Brochure

          Title
          Insect Cell Expression
          Overnight Express Autoinduction Systems
          Protein Purification and Preparation

          Citations

          Title
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        • Kepple, K/V., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 6867.
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        • Kumaran, D., et al. 2005. Protein Science 14, 719.
        • Meyn, M.A., et al. 2005. Molecular Pharmacology 68, 1320.
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        • Vuola, J.M., et al. 2005. Journal of Immunology 174, 449.
        • Wu, T., et al. 2005. Cell 121, 235.
        • Yamakoshi, Y., et al. 2005. Journal of Biological Chemistry 280, 1552.
        • Zanghi, C.N., et al. 2005. Nucleic Acids Research 33, e160.
        • Kasim, V., et al. 2004. Nucleic Acids Research 32, e66.
        • Lunin, et al. 2004. Journal of Biological Chemistry 279, 23422.
        • Moy, S., et al. 2004. Journal of Structural and Functional Genomics 5, 103.
        • Nguyen, H., et al. 2004. Journal of Structural and Functional Genomics 5, 23.
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        • User Protocols

          Title
          TB261 Benzonase<sup>®</sup> Nuclease

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