196800 | Bax Activator, BAM7 - Calbiochem

196800
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₂₁H₁₉N₅O₂S • HBr

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      196800-10MG
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          Glass bottle 10 mg
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          Description
          Overview

          This product has been discontinued.



          A cell-permeable pyrazolone compound that triggers Bax conformational changes by targeting Bax N-terminal face "BH3 trigger site" formed by the confluence of α-helices 1 &2 in a reversible manner (IC50 = 4.41 µM against 50 nM BIM BH3-derived peptide for Bax binding), leading to dose-dependent Bax oligomerization induction, Bax mitochondria translocation, and eventual Bax-dependent apoptotic cell death (33%, 90% and 94% survival rate in Bak-/-, Bak-/- Bax-/-, and Bax-/- MEF cultures, respectively; 10 µM for 24 h).

          Catalogue Number196800
          Brand Family Calbiochem®
          Synonyms(E)-4-(2-(2-Ethoxyphenyl)hydrazono)-3-methyl-1-(4-phenylthiazol-2-yl)-1H-pyrazol-5(4H)-one, HBr, Bax Activating Molecule 7
          References
          ReferencesGavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.
          Product Information
          FormOrange powder
          Hill FormulaC₂₁H₁₉N₅O₂S • HBr
          Chemical formulaC₂₁H₁₉N₅O₂S • HBr
          ReversibleY
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetBax
          Purity≥99% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Gavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-February-2013 JSW
          Synonyms(E)-4-(2-(2-Ethoxyphenyl)hydrazono)-3-methyl-1-(4-phenylthiazol-2-yl)-1H-pyrazol-5(4H)-one, HBr, Bax Activating Molecule 7
          DescriptionA cell-permeable pyrazolone compound that triggers Bax conformational changes by targeting Bax N-terminal face "BH3 trigger site" formed by the confluence of α-helices 1 &2 in a reversible manner (IC50 = 4.41 µM against 50 nM BIM BH3-derived FITC-BIM SAHB peptide for Bax binding), leading to dose-dependent Bax oligomerization induction, Bax mitochondria translocation, and eventual Bax-dependent apoptotic cell death (33%, 90% and 94% survival rate in Bak-/-, Bak-/- Bax-/-, and Bax-/- MEF cultures, respectively; 10 µM for 24 h). BAM7 is shown to exhibit selective affinity toward Bax, but not N-terminal BIM BH3 binding site in the proapoptotic Bak, or the antiapoptotic BCL-XL, MCL-1, and Bfl-1/A1. The N-terminal "Bax trigger site" is distinct from the canonical BH3-binding site at the C-terminal face of Bcl-2 family proteins and Bax K21E mutation is shown to abolish BAM7 binding.
          FormOrange powder
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₂₁H₁₉N₅O₂S • HBr
          Structure formulaStructure formula
          Purity≥99% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesGavathiotis, E., et al. 2012. Nat. Chem. Biol. 8, 639.