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71546 | BacMagic™ Transfection Kit - Novagen

71546
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      Overview

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      71546-3
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          Glass bottle 5 rxn
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          Description
          OverviewThe BacMagic™-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 Transfection Kit contains the BacMagic™-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector® Medium, for optimal transfection performance.
          Catalogue Number71546
          Brand Family Novagen®
          Application Data
          Figure 1. Construction of baculovirus recombinants with three BacMagic systems.

          Figure 2. Streamlined baculovirus method
          References
          Product Information
          0.5 µgBacMagic DNA
          50 µlInsect GeneJuice Transfection Reagent
          2 x 1 mlSf9 Cells
          1 LBacVector® Insect Cell Medium
          2 µgTransfection Control Plasmid
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Multiple Storage Conditions
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage Multiple storage requirements
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications