203638 | BMP-1/PCP, CF, Human, Recombinant

203638
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      203638-10UG
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      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
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          Glass bottle 10 μg
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          Description
          OverviewRecombinant, human bone morphogenetic protein (BMP-1) consisting of amino acids 121-730 and expressed in NS0-derived mouse myeloma cells with a C-terminal His•Tag® sequence. Will cleave a fluorogenic peptide substrate.
          Catalogue Number203638
          Brand Family Calbiochem®
          SynonymsProcollagen C-proteinase, PCP
          Application Data
          *Adjusted for Substrate Blank **Derived using calibration standard MCA-Pro-Leu-OH
          References
          ReferencesSteiglitz, B.M., et al. 2004. J. Biol. Chem. 279, 980.
          Hartigan, N., et al. 2003. J. Biol. Chem. 278, 18045.
          Leighton, M. and K.E. Kadler. 2003. J. Biol. Chem. 278, 18478.
          Bond, J.S. and R.J. Beynon. 1995. Protein Sci. 4, 1247.
          Wozney, J.M., et al. 1988. Science 242, 1528.
          Product Information
          FormSolid
          FormulationLyophilized from 0.2 µm filtered HEPES, NaCl, CaCl₂, ZnCl₂, and Brij®35 detergent.
          Applications
          Biological Information
          Biological activity>2 pmol/min/µg as determined by cleavage of a fluorogenic peptide substrate, Mca-YVADAPK (Dnp)-OH
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are good for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          203638

          References

          Reference overview
          Steiglitz, B.M., et al. 2004. J. Biol. Chem. 279, 980.
          Hartigan, N., et al. 2003. J. Biol. Chem. 278, 18045.
          Leighton, M. and K.E. Kadler. 2003. J. Biol. Chem. 278, 18478.
          Bond, J.S. and R.J. Beynon. 1995. Protein Sci. 4, 1247.
          Wozney, J.M., et al. 1988. Science 242, 1528.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-September-2011 RFH
          SynonymsProcollagen C-proteinase, PCP
          Application Data
          *Adjusted for Substrate Blank **Derived using calibration standard MCA-Pro-Leu-OH
          DescriptionRecombinant, human bone morphogenetic protein (BMP-1) consisting of amino acids 121-730 and expressed in NS0-derived mouse myeloma cells with a C-terminal His•Tag® sequence.
          FormSolid
          FormulationLyophilized from 0.2 µm filtered HEPES, NaCl, CaCl₂, ZnCl₂, and Brij®35 detergent.
          Recommended reaction conditions

          Activity Assay Protocol:
          Materials:
          Assay Buffer: 25 mM HEPES, 0.1% Brij®35 detergent (v/v), pH 7.5
          Recombinant human BMP-1
          Fluorogenic Peptide Substrate: MCA-Tyr-Val-Ala-Asp-Ala-Pro-Lys( DNP)-OH
          F16 Black Maxisorp Plate
          Fluorescent Plate Reader

          Assay:
          1. Dilute rhBMP-1 to 20 ng/µl in Assay Buffer.
          2. Dilute substrate to 20 µM in Assay Buffer.
          3. Add 50 µl of diluted rhBMP-1 to designated wells of the black plate. Start the reaction by adding 50 µl diluted Substrate. Include a Substrate Blank that contains only Assay Buffer (in place of rhBMP-1).
          4. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 min.
          5. Calculate specific activity:

          Figure 1: Calculations

          *Adjusted for Substrate Blank **Derived using calibration standard MCA-Pro-Leu-OH

          Final Assay Conditions Per Well
          rhBMP-1: 1 µg
          Substrate: 10 µM
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Biological activity>2 pmol/min/µg as determined by cleavage of a fluorogenic peptide substrate, Mca-YVADAPK (Dnp)-OH
          SolubilityReconstitute to 100 µg/ml in sterile 25 mM HEPES and 0.01% Brij35, pH 7.5.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are good for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesSteiglitz, B.M., et al. 2004. J. Biol. Chem. 279, 980.
          Hartigan, N., et al. 2003. J. Biol. Chem. 278, 18045.
          Leighton, M. and K.E. Kadler. 2003. J. Biol. Chem. 278, 18478.
          Bond, J.S. and R.J. Beynon. 1995. Protein Sci. 4, 1247.
          Wozney, J.M., et al. 1988. Science 242, 1528.