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69956 | BLR(DE3)pLysS Competent Cells - Novagen

69956
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      Overview

      Replacement Information

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      69956-3
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          Glass bottle 0.4 ml
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          69956-4
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              Glass bottle 1 ml
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              Description
              OverviewBLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

              pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
              Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) Δ(srl-recA)306::Tn10 pLysS (CamR, TetR)
              Catalogue Number69956
              Brand Family Novagen®
              References
              References1. A. Roca (U. of Wisconsin), personal
              communication.

              2. Studier, F. W. (1991) J. Mol. Biol. 219,
              37-44.
              Product Information
              Components
              Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/μg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
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              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              69956

              References

              Reference overview
              1. A. Roca (U. of Wisconsin), personal
              communication.

              2. Studier, F. W. (1991) J. Mol. Biol. 219,
              37-44.

              Brochure

              Title
              Competent Cell Brochure

              Citations

              Title
            • Min-Ju Kim, et al. (2007) NMR structural studies of interactions of a small, nonpeptidyl Tpo mimic with the thrombopoietin receptor extracellular juxtamembrane and transmembrane domains. Journal of Biological Chemistry 282, 14253-14261.
            • Anthony N. Anselmo, et al. (2006) WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Procedings of the National Academy of Science 103, 10883-10888.
            • Irina Bruck, Roxana E. Georgescu and Mike O'Donnell. (2005) Conserved interactions in the Staphylococcus aureus DNA polC chromosome replication machine. Journal of Biological Chemistry 280, 18152-18162.
            • C. Michael Reynolds, et al. (2005) A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. identification of the eptB gene and Ca++ hypersensitivity of an eptB deletion mutant. Journal of Biological Chemistry 280, 21202-21211.
            • An X. Tran, et al. (2004) Periplasmic cleavage and modification of the 1-phosphate group of Helicobacter pylori lipid A. Journal of Biological Chemistry 279, 55780-55791.
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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression