70235 BL21(DE3) Singles™ Competent Cells - Novagen

70235
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      Overview

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      70235-3
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          Glass bottle 11 rxn
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          70235-4
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              Glass bottle 22 rxn
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              Description
              OverviewA common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen® Singles™ Competent Cells are designed for ultimate convenience and reliability in plasmid transformation. Chemically competent cells are prepared using an optimized method. Cells are provided in 50 µl volumes, eliminating the need to aliquot, freeze/thaw, or waste partially used vials, saving time and money and ensuring reliable cell performance. To use, simply thaw, add DNA, incubate 5 minutes on ice, heat shock for 30 seconds, place on ice for 2 minutes, and add SOC medium.
              T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
              BL21 has been the gold standard for protein expression since it was first introduced in 1990. Deficient in lon and ompT proteases, BL21 and its derivatives are ideal for many applications.
              Genotype: F ompT hsdSB (rB mB) gal dcm (DE3)


              This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




              This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
              Catalogue Number70235
              Brand Family Novagen®
              References
              Product Information
              11 rxn or 22 rxnBL21(DE3) Singles Competent Cells
              2 or 4 × 2 mlSOC Medium
              10 µlTest Plasmid
              GenotypeF ompT hsdSB (rB mB) gal dcm (DE3)
              Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁷ cfu/μg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              BL21(DE3) Singles™ Competent Cells - Novagen SDS

              Title

              Safety Data Sheet (SDS) 

              BL21(DE3) Singles™ Competent Cells - Novagen Certificates of Analysis

              TitleLot Number
              70235

              Brochure

              Title
              The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

              Citations

              Title
            • Pokidysheva, E., et al. 2006. Cell 124, 485.
            • Bachler, C., et al. 2005. European Molecular Biology Organization Journal 24, 283.
            • Birgander, P.L., et al. 2005. Journal of Biological Chemistry 280, 14997.
            • Alivan Bueren, A.L., et al. 2005. Journal of Biological Chemistry 280, 530.
            • Chen, M-H., et al. 2005. Journal of Biological Chemistry 280, 10599.
            • Francis, K., et al. 2005. Journal of Biological Chemistry 280, 5195.
            • Hanaoka, S., et al. 2005. Protein Science 14, 119.
            • Hoffmeister, M., et al. 2005. Journal of Biological Chemistry 280, 4329.
            • Hosoya-Matsuda, N., et al. 2005. Journal of Biological Chemistry 280, 840.
            • Hoyos, B., et al. 2005. Journal of Biological Chemistry 280, 6872.
            • Jarvinen, A., et al. 2005. Journal of Biological Chemistry 280, 6595.
            • Kani, K., et al. 2005. Journal of Biological Chemistry 280, 8238.
            • Krug, A., et al. 2005. Journal of Biological Chemistry 280, 585.
            • Lo, K.W.-H., et al. 2005. Journal of Biological Chemistry 280, 8172.
            • Murphy, H.N., et al. 2005. Journal of Biological Chemistry 280, 14524.
            • Nakamaru-Ogiso, E., et al. 2005. Journal of Biological Chemistry 280, 301.
            • Sacchetto, R., et al. 2005. Journal of Biological Chemistry 280, 7147.
            • Smith, J.J., et al. 2005. Protein Science 14, 64.
            • Chen, Y-H., et al. 2004. Nature 429, 675.
            • Codina, A., et al. 2004 Journal of Biological Chemistry 279, 53338.
            • Im, Y.J., et al. 2004. Journal of Biological Chemistry 279, 53451.
            • Katona, L.I., et al. 2004. Journal of Bacteriology 186, 6443.
            • Parker, J.S., et al. 2004. European Molecular Biology Organization Journal 23, 4727.
            • Sivendran, S., et al. 2004. Journal of Biological Chemistry 279, 53789.
            • Wower, I.K., 2004. Journal of Biological Chemistry 279, 54202.
            • User Protocols

              Title
              TB055 pET System Manual

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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression